T cell receptor alpha constant (TRAC) Recombinant Protein | TRAC recombinant protein

Recombinant Human T cell receptor alpha constant (TRAC)

Cat. Num. AAA957674

• Gene Names: TRAC; TRA; IMD7; TCRA; TRCA
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: T cell receptor alpha constant (TRAC); Recombinant Human T cell receptor alpha constant (TRAC); TRAC recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-142. Full-Length
• Sequence: PNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Product Categories/Family for TRAC recombinant protein

Immunology

References

The chromosomal location of T-cell receptor genes and a T cell rearranging gene possible correlation with specific translocations in human T cell leukaemia.Rabbitts T.H., Lefranc M.P., Stinson M.A., Sims J.E., Schroder J., Steinmetz M., Spurr N.L., Solomon E., Goodfellow P.N.EMBO J. 4:1461-1465(1985) Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line.Yanagi Y., Chan A., Chin B., Minden M., Mak T.W.Proc. Natl. Acad. Sci. U.S.A. 82:3430-3434(1985) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.Nat. Biotechnol. 27:378-386(2009) Mutation in the TCRalpha subunit constant gene (TRAC) leads to a human immunodeficiency disorder characterized by a lack of TCRalphabeta+ T cells.Morgan N.V., Goddard S., Cardno T.S., McDonald D., Rahman F., Barge D., Ciupek A., Straatman-Iwanowska A., Pasha S., Guckian M., Anderson G., Huissoon A., Cant A., Tate W.P., Hambleton S., Maher E.R.J. Clin. Invest. 121:695-702(2011) The 1.5 A crystal structure of a highly selected antiviral T cell receptor provides evidence for a structural basis of immunodominance.Kjer-Nielsen L., Clements C.S., Brooks A.G., Purcell A.W., McCluskey J., Rossjohn J.Structure 10:1521-1532(2002) A structural basis for immunodominant human T cell receptor recognition.Stewart-Jones G.B.E., McMichael A.J., Bell J.I., Stuart D.I., Jones E.Y.Nat. Immunol. 4:657-663(2003) Structure of a human autoimmune TCR bound to a myelin basic protein self-peptide and a multiple sclerosis-associated MHC class II molecule.Li Y., Huang Y., Lue J., Quandt J.A., Martin R., Mariuzza R.A.EMBO J. 24:2968-2979(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 135511
• NCBI GeneID: 28755
• UniProt Accession #: P01848
• Molecular Weight: 17.9 kDa
• NCBI Official Full Name: T-cell receptor alpha chain C region
• NCBI Official Synonym Full Names: T-cell receptor alpha constant
• NCBI Official Symbol: TRAC
• NCBI Official Synonym Symbols: TRA; IMD7; TCRA; TRCA
• UniProt Protein Name: T-cell receptor alpha chain C region
• Protein Family: T-cell receptor
• UniProt Gene Name: TRAC
• UniProt Synonym Gene Names: TCRA
• UniProt Entry Name: TCA_HUMAN

NCBI Description

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Aug 2016]

Uniprot Description

MiscellaneousThis clone was isolated from a human leukemic T-cell line, Jurkat.