Mouse anti-Human CCL5 / RANTES Monoclonal Antibody | anti-CCL5 antibody

Mouse Monoclonal [clone 8.PP.11] (IgG2b) to Human CCL5 / RANTES

Cat. Num. AAA245262

• Gene Names: CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Neutralization Assay
• Purity: Protein A Purified
• Synonyms: CCL5 / RANTES; Monoclonal Antibody; Mouse Monoclonal [clone 8.PP.11] (IgG2b) to Human CCL5 / RANTES; Anti-CCL5 / RANTES Antibody (clone 8.PP.11) IHC-plus; CCL5; Chemokine (C-C motif) ligand 5; D17S136E; EoCP; T-cell specific protein p288; SCYA5; SISd; T cell-specific protein P228; Beta-chemokine RANTES; C-C motif chemokine 5; RANTES; SIS-delta; Small-inducible cytokine A5; Human CCL5; anti-CCL5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8.PP.11
• Specificity: Recognizes human RANTES.
• Purity/Purification: Protein A Purified
• Form/Format: PBS
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 91

• Applicable Applications for anti-CCL5 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA), Neutralization
• Application Notes: ELISA (2 - 4 ug/ml), IHC-P (5 ug/ml), Neut; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: RANTES.
• Immunogen: CCL5 / RANTES antibody was raised against rANTES.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-CCL5 antibody

Chemokine (C-C Motif) Ligand 5 (CCL5) is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils.

Product Categories/Family for anti-CCL5 antibody

Family: Intercrine

NCBI and Uniprot Product Information

• NCBI GI #: 22538814
• NCBI GeneID: 6352
• NCBI Accession #: NP_002976.2
• NCBI GenBank Nucleotide #: NM_002985.2
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99
• Molecular Weight: 9,990 Da
• NCBI Official Full Name: C-C motif chemokine 5 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• NCBI Protein Information: C-C motif chemokine 5; beta-chemokine RANTES; T-cell specific protein p288; eosinophil chemotactic cytokine; small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5; TCP228
• UniProt Entry Name: CCL5_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein homodimerization activity; protein self-association; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; protein binding; CCR1 chemokine receptor binding; CCR4 chemokine receptor binding; chemokine activity; chemokine receptor binding; chemokine receptor antagonist activity; phospholipase activator activity; CCR5 chemokine receptor binding; chemoattractant activity

Biological Process: regulation of chronic inflammatory response; exocytosis; positive regulation of cell adhesion; response to toxin; positive regulation of smooth muscle cell proliferation; positive regulation of JAK-STAT cascade; chemotaxis; positive regulation of smooth muscle cell migration; positive regulation of cell-cell adhesion mediated by integrin; protein amino acid phosphorylation; positive regulation of homotypic cell-cell adhesion; positive regulation of translational initiation; monocyte chemotaxis; leukocyte adhesion; positive chemotaxis; cell-cell signaling; calcium ion transport; positive regulation of innate immune response; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; dendritic cell chemotaxis; positive regulation of T cell proliferation; inflammatory response; protein tetramerization; phospholipase D activation; positive regulation of viral genome replication; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; response to virus; MAPKKK cascade; positive regulation of cellular biosynthetic process; macrophage chemotaxis; cellular calcium ion homeostasis; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; cellular protein complex assembly; negative regulation of viral genome replication; positive regulation of tyrosine phosphorylation of STAT protein; eosinophil chemotaxis; positive regulation of calcium ion transport; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; regulation of T cell activation