Mouse anti-Human Fas, Neutralizing Monoclonal Antibody
Fas, Neutralizing (CD95, APO-1)
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Neutralization Assay
Purity
Affinity PurifiedPurified by ammonium sulfate precipitation and Protein A affinity chromatography.
Synonyms
Fas; Neutralizing; Monoclonal Antibody; Neutralizing (CD95; APO-1); Anti -Fas; anti-Fas; Neutralizing antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.T.46
Specificity
Recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. Does not induce apoptosis in cell culture. Species Crossreactivity: Does not react with TNF and mouse.
Purity/Purification
Affinity Purified
Purified by ammonium sulfate precipitation and Protein A affinity chromatography.
Purified by ammonium sulfate precipitation and Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 50% glycerol.
Applicable Applications for anti-Fas, Neutralizing antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Neutralization
Application Notes
Suitable for use in Neutralization, Flow Cytometry and Western Blot.
Dilution: Neutralization: 500ng/ml neutralized over 50% of the apoptosis induced by F0019-64 Human Fas, Activating, treatment of human Jurkat cells.
Western Blot: 5ug/ml.
Flow Cytometry: 5-20ug/ml
Dilution: Neutralization: 500ng/ml neutralized over 50% of the apoptosis induced by F0019-64 Human Fas, Activating, treatment of human Jurkat cells.
Western Blot: 5ug/ml.
Flow Cytometry: 5-20ug/ml
Immunogen
Recombinant human Fas.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Fas, Neutralizing antibody
Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This "cellular suicide" can be mediated by the Fas antigen (CD95, APO1). Fas antigen is a cell-surface glycoprotein (40-50kD). It belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease. This mutation is a defect in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells.
Product Categories/Family for anti-Fas, Neutralizing antibody
Similar Products
Product Notes
The Fas, Neutralizing (Catalog #AAA601701) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fas, Neutralizing (CD95, APO-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fas, Neutralizing can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Neutralization. Suitable for use in Neutralization, Flow Cytometry and Western Blot. Dilution: Neutralization: 500ng/ml neutralized over 50% of the apoptosis induced by F0019-64 Human Fas, Activating, treatment of human Jurkat cells. Western Blot: 5ug/ml. Flow Cytometry: 5-20ug/ml. Researchers should empirically determine the suitability of the Fas, Neutralizing for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fas, Neutralizing, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
