Mouse CD19 Monoclonal Antibody | anti-CD19 antibody

MOUSE ANTI HUMAN CD19

Cat. Num. AAA212806

• Gene Names: CD19; B4; CVID3
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: CD19; Monoclonal Antibody; MOUSE ANTI HUMAN CD19; anti-CD19 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LE-CD19
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 557

• Applicable Applications for anti-CD19 antibody: ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.; Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Maximum Dilution: Reducing conditions
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Histology Positive Control Tissue: Tonsil; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining formalin-fixed, paraffin-embedded human tonsil with Mouse anti Human CD19)

Testing Data

(Western blot analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate probed with Mouse anti Human CD19 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

Related Product Information for anti-CD19 antibody

Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region. Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and prloliferation. B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004) Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.

NCBI and Uniprot Product Information

• NCBI GI #: 296010921
• NCBI GeneID: 930
• NCBI Accession #: NP_001171569.1
• NCBI GenBank Nucleotide #: NM_001178098.1
• UniProt Accession #: P15391; Q96S68; Q9BRD6; A0N0P9; F5H635
• Molecular Weight: 61,200 Da
• NCBI Official Full Name: B-lymphocyte antigen CD19 isoform 1
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3
• NCBI Protein Information: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19
• UniProt Protein Name: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: CD19
• UniProt Entry Name: CD19_HUMAN

NCBI Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor signaling protein activity

Biological Process: epidermal growth factor receptor signaling pathway; B cell receptor signaling pathway; regulation of immune response; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3