Western Blot (WB)
(Figure 1: Western blot analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.)
Mouse FOXD3 Antibody | anti-FOXD3 antibody
FOXD3 Antibody
Gene Names
FOXD3; AIS1; HFH2; VAMAS2; Genesis
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
FOXD3; FOXD3 Antibody; AIS1; HFH2; Genesis; anti-FOXD3 antibody
Host
Mouse
Reactivity
Human, Mouse, Monkey
Isotype
IgG1
Clone Number
5G9
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
478
Applicable Applications for anti-FOXD3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human FOXD3 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Western Blot (WB)
(Figure 1: Western blot analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse mAb with DAB staining.)
Related Product Information for anti-FOXD3 antibody
FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development. This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development and is also required for trophoblast formation. FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3(-/-) mouse embryos fail around the time of implantation with loss of neural crest-derived structures. FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. Tissue specificity: Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Product Categories/Family for anti-FOXD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50kDa
NCBI Official Full Name
forkhead box protein D3
NCBI Official Synonym Full Names
forkhead box D3
NCBI Official Symbol
FOXD3
NCBI Official Synonym Symbols
AIS1; HFH2; VAMAS2; Genesis
NCBI Protein Information
forkhead box protein D3
UniProt Protein Name
Forkhead box protein D3
Protein Family
UniProt Gene Name
FOXD3
UniProt Synonym Gene Names
HFH2
NCBI Description
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. Mutations in this gene cause autoimmune susceptibility 1. [provided by RefSeq, Nov 2008]
Uniprot Description
Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Research Articles on FOXD3
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Product Notes
The FOXD3 foxd3 (Catalog #AAA850865) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FOXD3 Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's FOXD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the FOXD3 foxd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXD3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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