Western Blot (WB) (Western blotAll lanes: ZBTB6 antibody at 8ug/mlLane 1: Hela whole cell lysateLane 2: HepG-2 whole cell lysateLane 3: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1493419 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human heart tissue using MBS1493419 at dilution of 1:100)
Western Blot (WB) (All lanes: ZMYND10 antibody at 8ug/mlLane 1:A549 whole cell lysateLane 2:mouse kidney tissueLane 3:mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 51,50 kDaObserved band size: 50 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS1496585 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MBS1496585 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: ZNF699 antibody at 2ug/mlLane 1:jurkat cells Lane 2:K562 cellsLane 2:U87 cells SecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 74kDaObserved band size: 74kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7001399 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial cancer tissue using MBS7001399 at dilution 1:100)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Testis stained with Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Mesothelioma stained with Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).)
SDS-PAGE (SDS-PAGE AnalysisPurified Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).Confirmation of Purity and Integrity of Antibody)
Analysis of Protein Array containing more than 19,000 full-length human proteins using Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857). (Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Western Blot (WB) (Western blotAll lanes: ZNF562 antibody IgG at 12ug/ml+ 293T whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 49,41 kDaObserved band size: 49 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7002584 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7002584 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded MBS7045603 using human testis tissue at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7045603 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG-2 cells using MBS7045603 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: DBF4B antibody at 4ug/mlLane 1: MCF7 whole cell lysateLane 2: A549 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 68,48,55,19 kDaObserved band size: 47 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS1495733 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in:Mouse spleen tissueAll lanes: ZC4H2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 27,16,24,21,26 kDaObserved band size: 27 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7051244 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of Bcl-6 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). BCL6 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lymph tissue with Bcl-6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Bcl-6 Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blotAll lanes: ZNF785 antibody at 6.27ug/mlLane 1 : Hela whole cell lysateLane 2 : PC-3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 47,45 kDaObserved band size: 46,65,30 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7044179 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: ZNF133 antibody at 5ug/ml+ Hela whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 74,63,67 kDaObserved band size: 73 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7006150 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7006150 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: ZNF169antibody at 5.32ug/ml+ Hela whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 68 kDaObserved band size: 68,34 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7045977 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7045977 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: ZCCHC4 antibody at 0.6ug/ml+ A431 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60,26,59 kDaObserved band size: 54 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS1493930 at dilution of 1:100)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GLI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7004493 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7004493 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: ZNF264 antibody at 2.07ug/mlLane 1: Jurkat whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 71 kDaObserved band size: 71,19 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7044933 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7044933 at dilution 1:100)
Immunohistochemistry (IHC) (Anti-ZC3H12A / MCPIP1 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Western Blot (WB) (ZC12A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The ZC12A antibody detected the ZC12A protein (arrow).)
Flow Cytometry (FC/FACS) (ZC12A Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of Hela whole cell lysates, using UHRF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF) (MBS9607582 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9607582 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using UHRF1 Antibody. Lane 1: PC12 treated with blocking peptide; Lane 2: PC12; Lane 3: 293.)
SDS-Page (Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-4: NIH/3T3£¬Raji£¬PC-3 and Hepg2 cell, Primary antibody:GLI1 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
Western Blot (WB) (Western blot analysis of A20 expression in HeLa (A), Daudi (B), RAW264.7 (C) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of A20 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MID1 antibody (MBS9127519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using MID1 Rabbit pAb (MBS9127519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using MID1 antibody (MBS9127519). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (MBS9600271 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis on HuvEc cell lysate using ZNF387 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blotAll lanes: ZNF689 antibody at 5ug/mlLane 1: Hela whole cell lysateLane 2: HepG-2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 57 kDaObserved band size: 57 kDa)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2446).)
Testing Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using GATA-3 Mouse Monoclonal Antibody (GATA3/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Western Blot (WB) (Western blot analysis of GFI1B expression in Jurkat cell lysate;, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9603924 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WT1 antibody at 1:200 dilution.)
Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Molt-4; B: Raji) using a 10 % SDS PAGE gel and Wilms Tumor 1 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded RT2 xenograft using Wilms Tumor 1 antibody at a dilution of 1:500)