Rabbit BLIMP1 / PRDM1 Polyclonal Antibody | anti-BLIMP1 antibody

Rabbit Polyclonal to Human BLIMP1 / PRDM1

Cat. Num. AAA242181

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: BLIMP1 / PRDM1; Polyclonal Antibody; Rabbit Polyclonal to Human BLIMP1 / PRDM1; Anti-BLIMP1 / PRDM1 Antibody (C-Terminus) IHC-plus; PRDM1; BLIMP-1; BLIMP1; PRDI-binding factor-1; PRDI-BF1; PR domain-containing protein 1; PRDI-binding factor 1; Human BLIMP1; anti-BLIMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human PRDM1 / BLIMP-1 (carboxy terminus).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 825

• Applicable Applications for anti-BLIMP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (3 ug/ml), WB (0.5 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: 14 amino acid peptide from near the carboxy terminus of human Blimp-1 (GenBank accession no. NP_001189).
• Immunogen Type: Synthetic peptide
• Immunogen: BLIMP1 / PRDM1 antibody was raised against 14 amino acid peptide from near the carboxy terminus of human Blimp-1 (GenBank accession no. NP_001189).
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-BLIMP-1 antibody IHC of human colon, lymphoid follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Blimp-1 in Mouse Lung cells with Blimp-1 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 172072684
• NCBI GeneID: 639
• NCBI Accession #: NP_001189.2
• NCBI GenBank Nucleotide #: NM_001198.3
• UniProt Accession #: O75626; Q86WM7; B2REA6; E1P5E0
• Molecular Weight: 76,835 Da
• NCBI Official Full Name: PR domain zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1; BLIMP-1; PRDI-binding factor-1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive-regulatory domain I binding factor
• UniProt Protein Name: PR domain zinc finger protein 1
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1; PRDI-binding factor 1
• UniProt Entry Name: PRDM1_HUMAN

NCBI Description

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; protein binding; metal ion binding; histone deacetylase binding; transcription factor activity

Biological Process: methylation; negative regulation of lipopolysaccharide-mediated signaling pathway; eye photoreceptor cell development; cell fate commitment; transcription, DNA-dependent; positive regulation of B cell differentiation; negative regulation of B cell proliferation; negative regulation of transcription from RNA polymerase II promoter; maternal placenta development; germ cell development; post-embryonic development