Western Blot (WB)
(Western blot analysis of CXXC5 in human brain tissue lysate with CXXC5 antibody at 1 μg/mL.)
Rabbit CXXC5 Polyclonal Antibody | anti-CXXC5 antibody
CXXC5 Antibody
Gene Names
CXXC5; CF5; WID; RINF; HSPC195
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
CXXC5 Antibody is affinity chromatography purified via peptide column.
Synonyms
CXXC5; Polyclonal Antibody; CXXC5 Antibody; CF5; WID; RINF; HSPC195; CXXC-type zinc finger protein 5; Putative MAPK-activating protein PM08; CXXC finger protein 5; anti-CXXC5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of CXXC5 are known to exist. CXXC5 antibody is predicted to not cross-react with other CXXC protein family members.
Purity/Purification
CXXC5 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
322
Applicable Applications for anti-CXXC5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
CXXC5 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CXXC5.
Buffer
CXXC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
CXXC5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of CXXC5 in human brain tissue lysate with CXXC5 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of CXXC5 in human brain tissue lysate with CXXC5 antibody at 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of CXXC5 in human brain tissue with CXXC5 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of CXXC5 in human brain tissue with CXXC5 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of CXXC5 in Human Brain tissue with CXXC5 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of CXXC5 in Human Brain tissue with CXXC5 antibody at 20 μg/mL.)
Related Product Information for anti-CXXC5 antibody
CXXC5 Antibody: CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax), and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,072 Da
NCBI Official Full Name
CXXC-type zinc finger protein 5
NCBI Official Synonym Full Names
CXXC finger protein 5
NCBI Official Symbol
CXXC5
NCBI Official Synonym Symbols
CF5; WID; RINF; HSPC195
NCBI Protein Information
CXXC-type zinc finger protein 5; CXXC finger 5 protein; retinoid-inducible nuclear factor; WT1-induced Inhibitor of Dishevelled; putative MAPK-activating protein PM08; putative NF-kappa-B-activating protein 102
UniProt Protein Name
CXXC-type zinc finger protein 5
Protein Family
UniProt Gene Name
CXXC5
UniProt Synonym Gene Names
CF5; RINF
UniProt Entry Name
CXXC5_HUMAN
Uniprot Description
CXXC5: May indirectly participate in activation of the NF- kappa-B and MAPK pathways. Acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells. Required for DNA damage-induced ATM phosphorylation, p53 activation and cell cycle arrest. Involved in myelopoiesis. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: signal transducer activity; DNA binding; zinc ion binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction
Research Articles on CXXC5
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Product Notes
The CXXC5 cxxc5 (Catalog #AAA150962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CXXC5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CXXC5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CXXC5 cxxc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXXC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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