Rabbit anti-Human ZC3H12A / MCPIP1 Polyclonal Antibody | anti-ZC3H12A / MCPIP1 antibody

Anti-ZC3H12A / MCPIP1 Antibody (aa331-358) IHC-plus

Cat. Num. AAA249619

• Gene Names: ZC3H12A; MCPIP; MCPIP1; dJ423B22.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: ZC3H12A / MCPIP1; Polyclonal Antibody; Anti-ZC3H12A / MCPIP1 Antibody (aa331-358) IHC-plus; Rabbit Polyclonal to Human ZC3H12A / MCPIP1; Human ZC3H12A / MCPIP1; ZC3H12A; DJ423B22.1; MCPIP; Ribonuclease ZC3H12A; MCP induced protein 1; MCP-induced protein 1; MCPIP1; RP3-423B22.1; anti-ZC3H12A / MCPIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This ZC12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the Central region of human ZC12A.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 599

• Applicable Applications for anti-ZC3H12A / MCPIP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:10 - 1:50); IHC-P (1:100); WB (1:1000); ; Optimal dilutions to be determined by the researcher.
• Immunogen Species: ZC3H12A/MCPIP1 antibody was raised against Human
• Antigen Modification: aa331-358
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-ZC3H12A / MCPIP1 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(ZC12A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The ZC12A antibody detected the ZC12A protein (arrow).)

Flow Cytometry (FC/FACS)

(ZC12A Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ZC3H12A / MCPIP1 antibody

ZC3H12A Antibody, DJ423B22.1 Antibody, MCPIP Antibody, Ribonuclease ZC3H12A Antibody, MCP induced protein 1 Antibody, MCP-induced protein 1 Antibody, MCPIP1 Antibody, RP3-423B22.1 Antibody Description: Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.

NCBI and Uniprot Product Information

• NCBI GI #: 156151383
• NCBI GeneID: 80149
• NCBI Accession #: NP_079355.2
• NCBI GenBank Nucleotide #: NM_025079.2
• UniProt Accession #: Q5D1E8; Q6I9Z1; Q9H5P1; D3DPT0
• NCBI Official Full Name: ribonuclease ZC3H12A
• NCBI Official Synonym Full Names: zinc finger CCCH-type containing 12A
• NCBI Official Symbol: ZC3H12A
• NCBI Official Synonym Symbols: MCPIP; MCPIP1; dJ423B22.1
• NCBI Protein Information: ribonuclease ZC3H12A
• UniProt Protein Name: Ribonuclease ZC3H12A
• UniProt Gene Name: ZC3H12A
• UniProt Entry Name: ZC12A_HUMAN

NCBI Description

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]

Uniprot Description

MCPIP1: Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA. Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. Belongs to the ZC3H12 family.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytoplasm; cytoskeleton; nucleoplasm; nucleus; plasma membrane; rough endoplasmic reticulum

Molecular Function: chromatin binding; DNA binding; endoribonuclease activity; exoribonuclease activity; metal ion binding; miRNA binding; mRNA 3'-UTR binding; mRNA binding; protein binding; ribonuclease activity; RNA binding

Biological Process: angiogenesis; apoptosis; cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-6 production; negative regulation of macrophage activation; negative regulation of NF-kappaB import into nucleus; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of defense response to virus by host; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; protein oligomerization