Rabbit anti-Human DBF4B Polyclonal Antibody | anti-DBF4B antibody

DBF4B Antibody

Cat. Num. AAA1495733

• Gene Names: DBF4B; DRF1; ASKL1; CHIFB; ZDBF1B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: DBF4B; Polyclonal Antibody; DBF4B Antibody; Protein DBF4 homolog B; ASKL1; DRF1; Activator of S phase kinase-like protein 1; ASK-like protein 1; Chiffon homolog B; Dbf4-related factor 1; anti-DBF4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 431

• Applicable Applications for anti-DBF4B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Protein DBF4 homolog B protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: DBF4B antibody at 4ug/mlLane 1: MCF7 whole cell lysateLane 2: A549 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 68,48,55,19 kDaObserved band size: 47 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS1495733 at dilution of 1:100)

Related Product Information for anti-DBF4B antibody

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 307133739
• NCBI GeneID: 80174
• NCBI Accession #: NP_079380.2
• NCBI GenBank Nucleotide #: NM_025104.3
• UniProt Accession #: Q8NFT6; Q8TEX0; Q96B19; Q9H912; D3DX56
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: protein DBF4 homolog B isoform 2
• NCBI Official Synonym Full Names: DBF4 zinc finger B
• NCBI Official Symbol: DBF4B
• NCBI Official Synonym Symbols: DRF1; ASKL1; CHIFB; ZDBF1B
• NCBI Protein Information: protein DBF4 homolog B
• UniProt Protein Name: Protein DBF4 homolog B
• Protein Family: Protein
• UniProt Gene Name: DBF4B
• UniProt Synonym Gene Names: ASKL1; DRF1; ASK-like protein 1
• UniProt Entry Name: DBF4B_HUMAN

NCBI Description

This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and, in complex with the cell division cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

DBF4B: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q21.31|17q21

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: nucleic acid binding; protein binding; protein kinase activator activity; protein kinase binding; zinc ion binding

Biological Process: activation of protein kinase activity; cell cycle; positive regulation of cell proliferation