Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Mouse CD29 Monoclonal Antibody | anti-CD29 antibody
Mouse Anti Human CD29: FITC
Does not react with: Rat, Mouse
Does not react with: Rat, Mouse
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and RPE conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on monocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and RPE conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on monocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD61 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD61 and RPE conjugated Mouse anti Human CD29 . All experiments performed on human blood gated on platelets in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD61 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD61 and RPE conjugated Mouse anti Human CD29 . All experiments performed on human blood gated on platelets in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Published Customer image:Mouse Anti Human CD28 antibody, clone 12G10 used for the identification of rabbit adipose stromal stem cells by flow cytometry.Image caption:Rabbit fat pad originate performing ASC in vitro. a Photomicrographs of fibroblastoid elements adhering to plastic after adipose tissue digestion (upper left panel) subsequently generating colonies (upper right panel) visualized by Crystal Violet staining (lower left panel) and reaching confluence after three passages (lower right panel Ph1, scale bar 100 mum). b Rabbit ASC cumulative population doubling after passage 3(P3). c Mean values +/- SEM of antigen expressed on rabbit ASC by FACS (left table); representative immunophenotypical characterizations of rabbit ASC with cells positive for CD29, CD49e and CD90 (isotype control in gray). d Adipogenic differentiation after 10 days of induction visualized by Oil Red O staining and a corresponding non-induced control (upper panels; scale bar 100 mum). Representative osteogenic differentiation visualized by Alizarin Red staining after 14 days of induction and a corresponding non-induced control (lower panels)From: Piccinno MS, Veronesi E, Loschi P, Pignatti M, Murgia A, Grisendi G, Castelli I, Bernabei D, Candini O, Conte P, Paolucci P, Horwitz EM, De Santis G, Iughetti L, Dominici M.Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.Apoptosis. 2013 Oct;18(10):1274-89.)
Testing Data
(Published Customer image:Mouse Anti Human CD28 antibody, clone 12G10 used for the identification of rabbit adipose stromal stem cells by flow cytometry.Image caption:Rabbit fat pad originate performing ASC in vitro. a Photomicrographs of fibroblastoid elements adhering to plastic after adipose tissue digestion (upper left panel) subsequently generating colonies (upper right panel) visualized by Crystal Violet staining (lower left panel) and reaching confluence after three passages (lower right panel Ph1, scale bar 100 mum). b Rabbit ASC cumulative population doubling after passage 3(P3). c Mean values +/- SEM of antigen expressed on rabbit ASC by FACS (left table); representative immunophenotypical characterizations of rabbit ASC with cells positive for CD29, CD49e and CD90 (isotype control in gray). d Adipogenic differentiation after 10 days of induction visualized by Oil Red O staining and a corresponding non-induced control (upper panels; scale bar 100 mum). Representative osteogenic differentiation visualized by Alizarin Red staining after 14 days of induction and a corresponding non-induced control (lower panels)From: Piccinno MS, Veronesi E, Loschi P, Pignatti M, Murgia A, Grisendi G, Castelli I, Bernabei D, Candini O, Conte P, Paolucci P, Horwitz EM, De Santis G, Iughetti L, Dominici M.Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.Apoptosis. 2013 Oct;18(10):1274-89.)
Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin beta1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998).
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.; Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 10p11.2
Cellular Component: focal adhesion; cell surface; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; basement membrane; integrin complex; neuromuscular junction; sarcolemma; receptor complex; external side of plasma membrane; cleavage furrow; filopodium
Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; actin binding; alpha-actinin binding; protein kinase binding; peptide binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding
Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; axon extension; positive regulation of apoptosis; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; regulation of immune response; cell migration; in utero embryonic development; sarcomere organization; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; cellular defense response; homophilic cell adhesion; blood coagulation; leukocyte tethering or rolling; positive regulation of cell migration
Research Articles on CD29
Similar Products
Product Notes
The CD29 itgb1 (Catalog #AAA225641) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD29: FITC reacts with Human, Mink Does not react with: Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD29 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD29 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD29, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
