Mouse anti-Human TCERG1 Monoclonal Antibody | anti-TCERG1 antibody

Mouse monoclonal antibody Anti-Human TCERG1

Cat. Num. AAA120042

• Gene Names: TCERG1; Urn1; CA150; TAF2S
• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: TCERG1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TCERG1; transcription elongation regulator 1 (TCERG1); CA150; TAF2S; MGC133200; anti-TCERG1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: TCEAD79A
• Form/Format: Mouse monoclonal anti-human TCERG1 antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA)and 0.05% sodium azide (NaN ).
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 4233

• Applicable Applications for anti-TCERG1 antibody: ELISA (EIA), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 0.2-2 ug/ml; FC: 0.5-2 ug/sample; ICC: 2-100 ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TCERG1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of TCERG1 by Western blot.Samples: Whole cell lysate from human A2058 (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. TCEAD79A-2]Predicted molecular weight: 123 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TCERG1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. TCEAD79A-2])

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TCERG1 mAb. [Lot No. TCEAD79A-1])

Related Product Information for anti-TCERG1 antibody

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 91208416
• NCBI GeneID: 10915
• NCBI Accession #: NP_006697.2
• NCBI GenBank Nucleotide #: NM_006706
• Molecular Weight: 121,690 Da
• NCBI Official Full Name: Homo sapiens transcription elongation regulator 1 (TCERG1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription elongation regulator 1
• NCBI Official Symbol: TCERG1
• NCBI Official Synonym Symbols: Urn1; CA150; TAF2S
• NCBI Protein Information: transcription elongation regulator 1; TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD; TATA box-binding protein-associated factor 2S; co-activator of 150 kDa; transcription factor CA150
• UniProt Protein Name: Transcription elongation regulator 1
• Protein Family: Transcription elongation regulator
• UniProt Gene Name: TCERG1
• UniProt Entry Name: TCRG1_HUMAN

NCBI Description

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TCERG1: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Transcription factor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleolus; nucleus; actin cytoskeleton

Molecular Function: protein binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter