Rat DECTIN-1 Monoclonal Antibody | anti-DECTIN-1 antibody
RAT ANTI MOUSE DECTIN-1
Purified IgG - liquid
Immunohistology - Frozen: Application Note: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
Preparation
Fusion Partners
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
Testing Data
(Staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor: RPE (MBS215798))
Testing Data
(Staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor:FITC (MBS215794))
Testing Data
(Immunoperoxidase staining of a mouse skin cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor:Biotin (MBS215790))
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)
Testing Data
(Staining of mouse peripheral blood monocytes with Rat anti Mouse Beta-glucan Receptor (MBS215792))
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC (MBS215795))
NCBI and Uniprot Product Information
Uniprot Description
dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: zymosan receptor activity; opsonin binding; protein binding; metal ion binding; zymosan binding; carbohydrate binding; pattern recognition receptor activity; polysaccharide binding
Biological Process: immune system process; cell surface pattern recognition receptor signaling pathway; cell recognition; phagocytosis, recognition; pattern recognition receptor signaling pathway; response to molecule of fungal origin; positive regulation of tumor necrosis factor production; cell-cell adhesion; leukocyte activation during immune response; positive regulation of phagocytosis; innate immune response; response to yeast; phagocytosis, engulfment; detection of yeast; inflammatory response
