Mouse TOP1 Monoclonal Antibody | anti-TOP1 antibody

TOP1 Antibody (N-term)

Cat. Num. AAA9231643

• Gene Names: TOP1; TOPI
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Synonyms: TOP1; Monoclonal Antibody; TOP1 Antibody (N-term); DNA topoisomerase 1; DNA topoisomerase I; anti-TOP1 antibody

• Host: Mouse
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Specificity: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 765

• Applicable Applications for anti-TOP1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:1000; FC/FACS: 1:25
• Function: Releases the supercoiling and torsionaltension of DNA introduced during the DNAreplication and transcription by transientlycleaving and rejoining one strand of the DNAduplex. Introduces a single-strand break viatransesterification at a target site in duplexDNA. The scissile phosphodiester is attackedby the catalytic tyrosine of the enzyme,resulting in the formation of aDNA-(3'-phosphotyrosyl)-enzymeintermediate and the expulsion of a 5'-OHDNA strand. The free DNA strand then rotatesaround the intact phosphodiester bond on theopposing strand, thus removing DNAsupercoils. Finally, in the religation step, theDNA 5'-OH attacks the covalent intermediateto expel the active-site tyrosine and restorethe DNA phosphodiester backbone (Bysimilarity). Regulates the alternative splicingof tissue factor (F3) pre-mRNA in endothelialcells. Involved in the circadian transcription ofthe core circadian clock componentARNTL/BMAL1 by altering the chromatinstructure around the ROR response elements(ROREs) on the ARNTL/BMAL1 promoter.
• Cellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm.Note=Diffuse nuclear localization with someenrichment in nucleoli. On CPT treatment,cleared from nucleoli into nucleoplasm.Sumoylated forms found in both nucleoplasmand nucleoli.
• Tissue Location: Endothelial cells. Releases the supercoiling and torsional tensionof DNA introduced during the DNA replicationand transcription by transiently cleaving andrejoining one strand of the DNA duplex. Introduces a single-strand break viatransesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of aDNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OHDNA strand. The free DNA strand then undergoespassage around the unbroken strand thusremoving DNA supercoils. Finally, in thereligation step, the DNA 5'-OH attacks thecovalent intermediate to expel the active-sitetyrosine and restore the DNA phosphodiesterbackbone (By similarity). Regulates thealternative splicing of tissue factor (F3)pre-mRNA in endothelial cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from MCF-7,Jurkat,PC-12 cell line (from left to right), using TOP1 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells using TOP1 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

Related Product Information for anti-TOP1 antibody

Description: Purified Mouse Monoclonal Antibody (Mab)

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

References

D'Arpa P.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:2543-2547(1988). Kunze N.,et al.J. Biol. Chem. 266:9610-9616(1991). Ota T.,et al.Nat. Genet. 36:40-45(2004). Deloukas P.,et al.Nature 414:865-871(2001). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 11225260
• NCBI GeneID: 7150
• NCBI Accession #: NP_003277.1
• NCBI GenBank Nucleotide #: NP_003277.1
• UniProt Accession #: P11387
• Molecular Weight: H=91; M=91; Rat= 91kDa
• NCBI Official Full Name: DNA topoisomerase 1
• NCBI Official Synonym Full Names: DNA topoisomerase I
• NCBI Official Symbol: TOP1
• NCBI Official Synonym Symbols: TOPI
• NCBI Protein Information: DNA topoisomerase 1
• UniProt Protein Name: DNA topoisomerase 1
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP1
• UniProt Entry Name: TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Oncoprotein; Nucleolus; Isomerase; EC 5.99.1.2

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus

Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity

Biological Process: circadian rhythm; response to drug; chromatin remodeling; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation