Rabbit CD74 Antibody | anti-CD74 antibody

CD74 (B-Cell Marker)

Cat. Num. AAA4381446

• Gene Names: CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human, Baboon, Mouse; Does not react with Rat.
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD74; CD74 (B-Cell Marker); CLIP; DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADR-gamma (HLADG); Ia antigen-associated invariant chain; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA-DR gamma chain; anti-CD74 antibody

• Host: Rabbit
• Reactivity: Human, Baboon, Mouse; Does not react with Rat.
• Isotype: IgG
• Clone Number: CLIP/3127R
• Specificity: It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CD74 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 1-2ug/million cells; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: 293T, Daudi or Raji cells. Tonsil or lymph node.
• Immunogen: SU-DHL-4 lymphoma cells
• Cellular Localization: Cell Surface and paranuclear globular
• Hu-chromosome Location: 5q33.1
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).)

SDS-Page

(SDS-PAGE Analysis Purified CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R). Confirmation of Purity and Integrity of Antibody.)

Western Blot (WB)

(Western Blot Analysis of Human 293T cell lysate using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of Raji cells. CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R) followed by goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).)

References

1. Marder RJ et. al. Lab Invest 52: 497-504, 1985. 2. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.

NCBI and Uniprot Product Information

• NCBI GeneID: 972
• UniProt Accession #: P044233; P04233
• Molecular Weight: 33-41kDa
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; p33; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]