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Type
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Gene Name
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Application
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5464 results for "Secondary Antibodies Monoclonal" - showing 50-100
Western Blot (WB)
(All lanes : Anti-GAPDH Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: C6 whole cell lysatesLane 3: Hela whole cell lysatesLane 4: HUVEC whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GAPDH, Monoclonal Antibody (Cat# AAA9200139)
Full Name
GAPDH Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, mouse, rat
Applications
WB, EIA, IF, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot
(Anti-SRC Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)
SRC, Monoclonal Antibody (Cat# AAA9200273)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2037).)
gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody (Cat# AAA4380481)
Full Name
gp100/Melanosome/PMEL17/SILV (Melanoma Marker)
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human. Others not tested.
Applications
EIA, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)
Tau, Monoclonal Antibody (Cat# AAA8005577)
Full Name
Tau Antibody (pSer202/ pThr205), Clone AH36: Biotin
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human
Applications
WB, DB, ICC, IF, EIA
Purity
Affinity Purified
Pricing
$620/0.1 mg | $2,560/5x0.1 mg
Immunohistochemistry (IHC)
(MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)
CSF1R, Monoclonal Antibody (Cat# AAA9200563)
Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. MBS9200249 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Beta-Actin, Monoclonal Antibody (Cat# AAA9200249)
Full Name
Beta-Actin Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, mouse (Predicted Reactivity: Xenopus, Bovine, Chicken, Monkey, Pig, Rat)
Applications
WB, EIA, IF, IHC
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
SOX2, Monoclonal Antibody (Cat# AAA9200597)
Full Name
SOX2 Antibody
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Applications
WB, IF, IHC-P, FC/FACS
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2039).)
gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody (Cat# AAA4380483)
Full Name
gp100/Melanosome/PMEL17/SILV (Melanoma Marker)
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human. Others not tested.
Applications
EIA, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot
(Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D3A2 at 1ug/mL concentration.)
Beta 2 Microglobulin, Monoclonal Antibody (Cat# AAA592016)
Full Name
mAb anti-Human Beta 2 Microglobulin
Reactivity
Human. Others not tested
Applications
EIA
Purity
Protein G affinity purified
Pricing
$320/0.1 mg | $440/0.5 mg | $1,735/5x0.5 mg
Immunohistochemistry (IHC)
(Anti-CD4 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
CD4, Monoclonal Antibody (Cat# AAA249480)
Full Name
Anti-CD4 Antibody (clone 5D9) IHC-plus
Gene Names
CD4; CD4mut
Reactivity
Human, Monkey
Applications
IHC, IHC, WB
Purity
Protein A/G purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1. MBS9200054 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
UCHL1, Monoclonal Antibody (Cat# AAA9200054)
Full Name
UCHL1 Antibody
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human
Applications
EIA, IHC, IF, WB
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808952)
Full Name
Alpha Synuclein Antibody, Clone 3F8
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HUVEC cells using VEGFR3(green) compared to an isotype control of mouse IgG2a(blue). MBS9200212was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
VEGFR3, Monoclonal Antibody (Cat# AAA9200212)
Full Name
VEGFR3 Antibody
Gene Names
FLT4; PCL; FLT41; LMPH1A; VEGFR3
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot (WB)
(Western blot detection of MEK-2 in THP-1, Jurkat, Raji, 3T3 and Hela cell lysates using 310001 (MEK-2 rabbit pAb, 1:1000 diluted), then incubated with 201069 (Rabbit IgG mAb, 1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.)
IgG, Monoclonal Antibody (Cat# AAA475172)
Full Name
Anti-Rabbit IgG Mouse mAb
Reactivity
Rat
Applications
WB, EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Testing Data
(Fig-1: Expression analysis of Dc-Sign. Anti-Dc-Sign antibody (Clone: ABM47B7) was tested at 0.1 ug/ml on partial length recombinant protein.)
Dc-Sign/CD 209, Monoclonal Antibody (Cat# AAA668318)
Full Name
Monoclonal Antibody to Dc-Sign/CD 209 (Clone: ABM47B7)
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Fig-1: Western blot analysis of RANKL. Anti-RANKL antibody (Clone: ABM12D6) was used at 2 ug/ml on hHeart lysate.)
RANKL, Monoclonal Antibody (Cat# AAA668455)
Full Name
Monoclonal Antibody to RANKL (Clone: ABM12D6)
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Qa-1b in mouse thymus tissue lysate using MBS6007408 at 2ug/ml.)
Qa-1b, Monoclonal Antibody (Cat# AAA6007408)
Full Name
Qa-1b (H2 T23, H2 Class I Histocompatibility Antigen D37 alpha Chain, H2 Q1, Histocompatibility 2 T Region Locus 23, MHC Class I Antigen Qa1, MHC ClassIb Qa1, OTTMUSP00000015895, Qa1)
Reactivity
Mouse
Applications
WB, IP, FC
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$580/0.1 mg | $2,595/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214268)
Full Name
RAT ANTI MOUSE CD8:Low Endotoxin
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Immunohistochemistry (IHC)
( Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center). MBS9200125 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
PAH, Monoclonal Antibody (Cat# AAA9200125)
Full Name
PAH Antibody (Center)
Gene Names
PAH; PH; PKU; PKU1
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
SDS-Page
(SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to LAG-3 (LAG3/3261). Confirmation of Integrity and Purity of Antibody.)
Lymphocyte Activation Gene 3 (LAG-3), Monoclonal Antibody (Cat# AAA4381061)
Full Name
Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)
Gene Names
LAG3; CD223
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488486)
Full Name
Anti-TIGIT [1B4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
EIA, FC/FACS, BL
Purity
Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD3 epsilon and TRP1 antibodies. Mouse splenocytes (A), B16F10 murine melanoma cells (B), KPC3 pacreas carcinoma cells (C) and KPC3 cells transfected with the Trp1 gene (D) were fixed using 2% PFA, permeabilised using 0.5% Triton and were subject to a primary treatment of either buffer, mouse-IgG1 chimeric 2C11 or mouse-IgG1 chimeric TA99 (indicated above plots) before a secondary treatment with buffer, goat anti-mouse Ig-allophycocyanin (G-aM Ig-APC) or anti-HisTag-APC (aHis-APC) antibodies (indicated beside plots). In panel A, splenocytes were also stained with a commercially available anti-CD3 (2C11) antibody conjugated to phycoerythrin (PE); all cells (i-v) were CD3 and thus PE positive. In subpanel ‘A v’ an increase in APC fluorescence intensity (FI(APC)) indicates binding of aHis-APC to 2C11 bound to CD3 at the cell surface. Some Ig containing proteins expressed by the splenocytes may explain the increase in APC fluorescence in subpanel ‘A iii’. In panel B an increase in FI(APC) in subpanel ‘iii’ indicates that TA99 binds to heavily expressed TRP1 at B16F10 cell surfaces and is then detectable using an G-aM Ig-APC antibody. Conversely, G-aM Ig-APC did not detect 2C11 at the cell surface, whereas a subset of cells with 2C11 bound to the surface were detectable using aHis-APC. Panel C shows that TRP1 is not detectable in KPC3 carcinoma cells (‘Ci, iii, v’) as expected, and that again, aHis-APC is able to detect a small subset of CD3 expressing cells (‘C vi’). When transfected with the Trp1 gene, KPC3 cells then strongly express TRP1 and it becomes detectable (‘D iii’). A small subset of CD3 positive cells was again detectable in Trp1 transfected KPC3 cells (‘D vi’). All analyses were made using FACSCanto flow-cytometer.)
TRP-1, Monoclonal Recombinant Antibody (Cat# AAA488391)
Full Name
Anti-TRP-1, gp75 [TA99]
Gene Names
TYRP1; TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IL23A / IL-23 p19, Monoclonal Antibody (Cat# AAA246319)
Full Name
Mouse Monoclonal [clone HLT2736] (IgG1) to Human IL23A / IL-23 p19
Gene Names
IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
Reactivity
Human
Applications
IHC - Paraffin, WB
Purity
Affinity Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Western Blot (WB)
(All lanes : Anti-XRN1 Antibody at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
XRN1, Monoclonal Antibody (Cat# AAA9216876)
Full Name
XRN1 Antibody
Gene Names
XRN1; SEP1
Reactivity
Human
Applications
WB
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunohistochemistry (IHC)
(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))
FOLR1/Folate Receptor Alpha, Polyclonal Antibody (Cat# AAA2401574)
Full Name
Anti-FOLR1/Folate Receptor Alpha Antibody
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Flow Cytometry (FC)
(Flow cytometric analysis of U-87 MG cells using RAC1(green) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
RAC1, Monoclonal Antibody (Cat# AAA9200589)
Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Applications
WB, EIA, FC, IHC
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808971)
Full Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 390
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Test Data
(All lanes: Anti-CD81 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
CD81, Monoclonal Antibody (Cat# AAA9217050)
Full Name
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human
Applications
WB, EIA
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808972)
Full Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 488
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SLC3A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 25s.)
SLC3A2, Polyclonal Antibody (Cat# AAA9127600)
Full Name
SLC3A2 Polyclonal Antibody
Gene Names
SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-AXL antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
AXL, Monoclonal Antibody (Cat# AAA245668)
Full Name
Mouse Monoclonal [clone 7E10] (IgG1) to Human AXL
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human
Applications
IHC
Purity
Ascites
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).)
gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody (Cat# AAA4380482)
Full Name
gp100/Melanosome/PMEL17/SILV (Melanoma Marker)
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human. Others not tested.
Applications
EIA, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embeddedhuman Tonsil stained with CD25Mouse Monoclonal Antibody (IL2RA/2395).)
CD25/ IL2RA, Monoclonal Antibody (Cat# AAA4380450)
Full Name
CD25/ IL2RA (Activated Lymphocyte Marker)
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. skeletal muscle section using Musk Antibody. MBS9200303 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Musk, Monoclonal Antibody (Cat# AAA9200303)
Full Name
Musk Antibody
Gene Names
Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
Reactivity
Mouse, Rat
Applications
EIA, IHC, WB
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808981)
Full Name
Alpha Synuclein Antibody, Clone 4F1: Biotin
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
FCGR1A / CD64, Monoclonal Antibody (Cat# AAA249232)
Full Name
Anti-FCGR1A / CD64 Antibody (clone 3D3) IHC-plus
Gene Names
FCGR1A; CD64; FCRI; CD64A; IGFR1
Reactivity
Human
Applications
IHC, IF, WB
Purity
Protein A/G purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808974)
Full Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 594
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005608)
Full Name
VPS35 Antibody, Clone 7E4: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$520/0.1 mg | $2,135/5x0.1 mg
Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
HHEX / HEX, Monoclonal Antibody (Cat# AAA249287)
Full Name
Anti-HHEX / HEX Antibody (clone 3F4) IHC-plus
Gene Names
Hhex; Hex; Prh; Hex1; Prhx; Hhex-rs2
Reactivity
Human
Applications
IHC, IF
Purity
Protein A/G purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunofluorescence (IF)
(Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody. MBS9200345 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
CHRM2, Monoclonal Antibody (Cat# AAA9200345)
Full Name
CHRM2 Antibody
Gene Names
CHRM2; HM2
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data
(Nuclear expression of p63 visualized with MBS684194, anti-p63 antibody, clone I27-I; in normal hair follicles (A)basal cell carcinoma of the skin. )
p63, Monoclonal Antibody (Cat# AAA684194)
Full Name
Anti - p63
Gene Names
TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
Applications
IHC
Pricing
$375/7 mL (RTU) | $355/0.1 mL (Concentrate) | $580/0.5 mL (Concentrate) | $875/1 mL (Concentrate) | $465/0.2 mL (Concentrate) | $540/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,675/5x1 mL (Concentrate)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808790)
Full Name
Methylglyoxal Antibody, Clone 9E7
Applications
WB, ICC, IF, IHC, FC
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NEK2, Monoclonal Antibody (Cat# AAA245626)
Full Name
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human NEK2
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
IHC, IHC-P, WB, IP, EIA
Purity
Purified from ascites by protein A
Pricing
$650/0.05 mg | $2,875/5x0.05 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808803)
Full Name
Methylglyoxal Antibody, Clone 9E7: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808763)
Full Name
Malondialdehyde Antibody, Clone 6H6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808773)
Full Name
Malondialdehyde Antibody, Clone 11E3: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunohistochemistry (IHC)
(MBS9607213 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CD59, Polyclonal Antibody (Cat# AAA9607213)
Full Name
CD59 Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-IL-10 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IL10, Monoclonal Antibody (Cat# AAA246583)
Full Name
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL10
Gene Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Reactivity
Mouse
Applications
IHC, FC, EIA
Purity
Protein G Purified
Pricing
$585/0.1 mg | $2,585/5x0.1 mg
