Mouse anti-Human HHEX / HEX Monoclonal Antibody | anti-HHEX / HEX antibody

Anti-HHEX / HEX Antibody (clone 3F4) IHC-plus

Cat. Num. AAA249287

• Gene Names: Hhex; Hex; Prh; Hex1; Prhx; Hhex-rs2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Protein A/G purified
• Synonyms: HHEX / HEX; Monoclonal Antibody; Anti-HHEX / HEX Antibody (clone 3F4) IHC-plus; Mouse Monoclonal [clone 3F4] (IgG2b) to Human HHEX / HEX; Human HHEX / HEX; HMPH; Homeobox protein HEX; HOX11L-PEN; PRHX; Homeobox protein PRH; PRH; anti-HHEX / HEX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3F4
• Specificity: Human HHEX / HEX
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.5-1.0 mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-HHEX / HEX antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: IF (1:100); IHC-P (2.5 ug/ml); Optimal dilution to be determined by the researcher.
• Immunogen: HHEX / HEX antibody was raised against recombinant fragment expressed in E Coli corresponding to amino acids 100-270 of human Hex.
• Immunogen Description: Recombinant fragment expressed in E Coli corresponding to amino acids 100-270 of human Hex.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Anti-HHex mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HHex.)

Immunofluorescence (IF)

(Immunofluorescent staining of HT29 cells using anti-HHex mouse monoclonal antibody.)

Immunofluorescence (IF)

(Immunofluorescent staining of HeLa cells using anti-HHex mouse monoclonal antibody.)

Related Product Information for anti-HHEX / HEX antibody

HHEX Antibody, HEX Antibody, HMPH Antibody, Homeobox protein HEX Antibody, HOX11L-PEN Antibody, PRHX Antibody, Homeobox protein PRH Antibody, PRH Antibody Description: Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

NCBI and Uniprot Product Information

• NCBI GI #: 1170322
• NCBI GeneID: 15242
• UniProt Accession #: P43120; Q544N6; Q9CRV1; Q9R1X2
• NCBI Official Full Name: Hematopoietically-expressed homeobox protein Hhex
• NCBI Official Synonym Full Names: hematopoietically expressed homeobox
• NCBI Official Symbol: Hhex
• NCBI Official Synonym Symbols: Hex; Prh; Hex1; Prhx; Hhex-rs2
• NCBI Protein Information: hematopoietically-expressed homeobox protein Hhex
• UniProt Protein Name: Hematopoietically-expressed homeobox protein Hhex
• UniProt Gene Name: Hhex
• UniProt Synonym Gene Names: Prh; Prhx; Homeobox protein HEX; mHex
• UniProt Entry Name: HHEX_MOUSE

Uniprot Description

HHEX: Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

Protein type: Transcription factor; DNA-binding

Cellular Component: cytoplasm; nucleus

Molecular Function: chromatin binding; DNA bending activity; DNA binding; eukaryotic initiation factor 4E binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: anterior/posterior pattern formation; B cell differentiation; cell differentiation; cell proliferation; embryonic heart tube development; embryonic organ development; endoderm development; forebrain development; forebrain morphogenesis; hemopoiesis; in utero embryonic development; interkinetic nuclear migration; liver development; morphogenesis of an epithelium; mRNA export from nucleus; multicellular organism growth; multicellular organismal development; myeloid leukocyte differentiation; negative regulation of angiogenesis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of vascular endothelial growth factor receptor signaling pathway; Notch signaling pathway; organ morphogenesis; pancreas development; poly(A)+ mRNA export from nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; signal transduction; thyroid gland development; tissue morphogenesis; transcription, DNA-dependent; vasculogenesis; Wnt receptor signaling pathway