anti-Human NEK2 Monoclonal Antibody | anti-NEK2 antibody

Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human NEK2

Cat. Num. AAA245626

• Gene Names: NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Purified from ascites by protein A
• Synonyms: NEK2; Monoclonal Antibody; Mouse Monoclonal [clone 2F6] (IgG2a; k) to Human NEK2; Anti-NEK2 Antibody (clone 2F6) IHC-plus; NIMA-related kinase 2; NEK2A; HsPK21; NimA-like protein kinase 1; NLK1; HSPK 21; NimA-related protein kinase 2; Human NEK2; anti-NEK2 antibody

• Host: NEK2 antibody was produced in Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F6
• Specificity: Human NEK2
• Purity/Purification: Purified from ascites by protein A
• Form/Format: PBS, pH7.4

• Applicable Applications for anti-NEK2 antibody: Immunochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), Immunoprecipitaion (IP), ELISA (EIA)
• Application Notes: IHC - Paraffin (5 ug/ml)
• Target: NEK2
• Family: Protein Kinase/ NEK
• Immunogen Species: NEK 2 antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: NEK2 anitbody was raised against nEK2 (AAH43502, 331aa~445 aa) Partial recombinant protein with GST tag alone is 26KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short Term: 4°C; Long Term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.)

ELISA (EIA)

(Detection limit for recombinant GST tagged NEK2 is approximately 0.3 ng/ml as a capture antibody.)

Product Categories/Family for anti-NEK2 antibody

Family: Protein Kinase; Subfamily: NEK

NCBI and Uniprot Product Information

• NCBI GI #: 4505373
• NCBI GeneID: 4751
• NCBI Accession #: NP_002488.1
• NCBI GenBank Nucleotide #: NM_002497.3
• UniProt Accession #: P51955; Q53FD6; Q5I1Z9; Q5VXZ1; Q6NZX8; Q7Z634; Q86XH2; Q96QN9
• NCBI Official Full Name: serine/threonine-protein kinase Nek2 isoform 1
• NCBI Official Synonym Full Names: NIMA-related kinase 2
• NCBI Official Symbol: NEK2
• NCBI Official Synonym Symbols: NLK1; RP67; NEK2A; HsPK21; PPP1R111
• NCBI Protein Information: serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111; NIMA (never in mitosis gene a)-related kinase 2
• UniProt Protein Name: Serine/threonine-protein kinase Nek2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK2
• UniProt Synonym Gene Names: NEK2A; NLK1; NimA-related protein kinase 2
• UniProt Entry Name: NEK2_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Nucleolus; Kinase, protein; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: kinetochore; spindle pole; centrosome; microtubule; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; nucleolus; midbody; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; organelle organization and biogenesis; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle

Disease: Retinitis Pigmentosa 67