Mouse anti-Mouse, Rat Musk Monoclonal Antibody | anti-Musk antibody

Musk Antibody

Cat. Num. AAA9200303

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Reactivity: Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: Musk; Monoclonal Antibody; Musk Antibody; Muscle; skeletal receptor tyrosine-protein kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor; Nsk2; anti-Musk antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1429CT456.173.44
• Specificity: This mouse Musk antibody is generated from a mouse immunized with recombinant protein from mouse Musk.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 600-860
• Sequence Length: 878

• Applicable Applications for anti-Musk antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC~~1:25; WB~~1:2000
• Function: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
• Cellular Location: Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein. Note=Localizes to the postsynaptic cell membrane of the neuromuscular junction
• Tissue Location: Expressed preferentially in skeletal muscle.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded M. skeletal muscle section using Musk Antibody. MBS9200303 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from C2C12, L6 cell line (from left to right), using Musk Antibody. MBS9200303 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Related Product Information for anti-Musk antibody

Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.

Product Categories/Family for anti-Musk antibody

Crown Antibodies; Signal Transduction

References

Caruso A.,et al.Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
Ganju P.,et al.Oncogene 11:281-290(1995).
DeChiara T.M.,et al.Cell 85:501-512(1996).
Glass D.J.,et al.Cell 85:513-523(1996).
Lin W.,et al.Nature 410:1057-1064(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 82533041
• NCBI GeneID: 18198
• NCBI Accession #: NP_001032205.1
• NCBI GenBank Nucleotide #: NM_001037128.1
• UniProt Accession #: Q61006; Q61005; Q61987; Q61988
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muskelin
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; Musk family

Cellular Component: postsynaptic membrane; cell projection; membrane; integral to plasma membrane; plasma membrane; integral to membrane; synapse; neuromuscular junction; cell junction; receptor complex; external side of plasma membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; ATP binding; PDZ domain binding; protein kinase activity

Biological Process: positive regulation of synaptic transmission, cholinergic; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of synaptic growth at neuromuscular junction; signal transduction; protein amino acid phosphorylation; receptor clustering; memory; positive regulation of peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; cell differentiation; response to electrical stimulus; phosphorylation; neuromuscular junction development