Mouse anti-Human, mouse SRC Monoclonal Antibody | anti-SRC antibody

SRC Antibody

Cat. Num. AAA9200273

• Gene Names: SRC; ASV; SRC1; c-SRC; p60-Src
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunofluorescence
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: SRC; Monoclonal Antibody; SRC Antibody; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1; anti-SRC antibody

• Host: Mouse
• Reactivity: Human, mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 17AT28
• Specificity: This Src antibody is generated from mouse immunized with a recombinant protein of full length human SRC.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 536

• Applicable Applications for anti-SRC antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: WB~~1:100~500; IF~~1:10~50
• Function: Non-receptor protein tyrosine kinase which is activated following engagement of manydifferent classes of cellular receptorsincluding immune response receptors,integrins and other adhesion receptors,receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokinereceptors. Participates in signaling pathwaysthat control a diverse spectrum of biologicalactivities including gene transcription,immune response, cell adhesion, cell cycleprogression, apoptosis, migration, andtransformation. Due to functional redundancybetween members of the SRC kinase family,identification of the specific role of each SRCkinase is very difficult. SRC appears to be oneof the primary kinases activated followingengagement of receptors and plays a role inthe activation of other protein tyrosine kinase(PTK) families. Receptor clustering ordimerization leads to recruitment of SRC tothe receptor complexes where itphosphorylates the tyrosine residues withinthe receptor cytoplasmic domains. Plays animportant role in the regulation ofcytoskeletal organization throughphosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows theSRC SH2 domain to bind AFAP1 and tolocalize to actin filaments. Cytoskeletalreorganization is also controlled through thephosphorylation of cortactin (CTTN). Whencells adhere via focal adhesions to theextracellular matrix, signals are transmittedby integrins into the cell resulting in tyrosinephosphorylation of a number of focaladhesion proteins, including PTK2/FAK1 andpaxillin (PXN). In addition to phosphorylatingfocal adhesion proteins, SRC is also active atthe sites of cell-cell contact adherensjunctions and phosphorylates substrates suchas beta-catenin (CTNNB1), delta-catenin(CTNND1), and plakoglobin (JUP). Anothertype of cell-cell junction, the gap junction, isalso a target for SRC, which phosphorylatesconnexin- 43 (GJA1). SRC is implicated inregulation of pre-mRNA-processing andphosphorylates RNA-binding proteins such asKHDRBS1. Also plays a role in PDGF-mediatedtyrosine phosphorylation of both STAT1 andSTAT3, leading to increased DNA bindingactivity of these transcription factors.Involved in the RAS pathway throughphosphorylation of RASA1 and RASGRF1.Plays a role in EGF-mediatedcalcium-activated chloride channel activatiion. Required for epidermal growth factor receptor(EGFR) internalization throughphosphorylation of clathrin heavy chain (CLTCand CLTCL1) at 'Tyr- 1477'. Involved inbeta-arrestin (ARRB1 and ARRB2)desensitization through phosphorylation andactivation of ADRBK1, leading to beta-arrestin phosphorylation and internalization.Has a critical role in the stimulation of theCDK20/MAPK3 mitogen-activated proteinkinase cascade by epidermal growth factor.Might be involved not only in mediating thetransduction of mitogenic signals at the levelof the plasma membrane but also incontrolling progression through the cell cyclevia interaction with regulatory proteins in the nucleus. Plays an important role inosteoclastic bone resorption in conjunctionwith PTK2B/PYK2. Both the formation of aSRC-PTK2B/PYK2 complex and SRC kinaseactivity are necessary for this function.Recruited to activated integrins byPTK2B/PYK2, thereby phosphorylating CBL,which in turn induces the activation and recruitment of phosphatidylinositol 3-kinaseto the cell membrane in a signaling pathwaythat is critical for osteoclast function.Promotes energy production in osteoclasts byactivating mitochondrial cytochrome Coxidase. Phosphorylates DDR2 on tyrosineresidues, thereby promoting its subsequentautophosphorylation. Phosphorylates RUNX3and COX2 on tyrosine residues, TNK2 on'Tyr-284' and CBL on 'Tyr-731'. EnhancesDDX58/RIG-I-elicited antiviral signaling.Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373'and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-128'. Phosphorylates CBLC at multipletyrosine residues, phosphorylation at'Tyr-341' activates CBLC E3 activity.
• Cellular Location: Cell membrane. Mitochondrion inner membrane. Nucleus. Cytoplasm,cytoskeleton. Note=Localizes to focal adhesion sites following integrin engagement.Localization to focal adhesion sites requires myristoylation and the SH3 domain
• Tissue Location: Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot

(Anti-SRC Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)

Western Blot

(Anti-SRC Antibody at 1:500 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody. MBS9200273 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Immunofluorescence (IF)

(Fluorescent image of A549 cell stained with SRC Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).SRC immunoreactivity is localized to Cytoplasm significantly.)

Western Blot (WB)

(The anti-Src Mab is used in Western blot to detect Src in Jurkat cell lysate.)

Western Blot (WB)

(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (MBS9200273) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

Related Product Information for anti-SRC antibody

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-SRC antibody

Crown Antibodies; Cancer; Signal Transduction

References

Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface. Dergai M, et al. Biochem Biophys Res Commun, 2010 Aug 20. PMID 20659428. Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via association with Src. Liu H, et al. J Biol Chem, 2010 Jul 16. PMID 20460377. Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner. Pene-Dumitrescu T, et al. J Biol Chem, 2010 Jul 9. PMID 20452982. Hydrogen peroxide activates focal adhesion kinase and c-Src by a phosphatidylinositol 3 kinase-dependent mechanism and promotes cell migration in Caco-2 cell monolayers. Basuroy S, et al. Am J Physiol Gastrointest Liver Physiol, 2010 Jul. PMID 20378826. Protein kinase G type Ialpha activity in human ovarian cancer cells significantly contributes to enhanced Src activation and DNA synthesis/cell proliferation. Leung EL, et al. Mol Cancer Res, 2010 Apr. PMID 20371672.

NCBI and Uniprot Product Information

• NCBI GI #: 4885609
• NCBI GeneID: 6714
• NCBI Accession #: NP_005408.1; NP_938033.1
• NCBI GenBank Nucleotide #: NM_005417.4
• UniProt Accession #: P12931; Q76P87; Q86VB9; Q9H5A8; E1P5V4
• Molecular Weight: 59835
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase Src
• NCBI Official Synonym Full Names: SRC proto-oncogene, non-receptor tyrosine kinase
• NCBI Official Symbol: SRC
• NCBI Official Synonym Symbols: ASV; SRC1; c-SRC; p60-Src
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase Src
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase Src
• Protein Family: Src-like-adapter
• UniProt Gene Name: SRC
• UniProt Synonym Gene Names: SRC1; p60-Src
• UniProt Entry Name: SRC_HUMAN

NCBI Description

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 20q12-q13

Cellular Component: extrinsic to internal side of plasma membrane; neuron projection; mitochondrion; lysosome; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; postsynaptic density; late endosome; plasma membrane; actin filament; caveola; nucleus; cytosol

Molecular Function: protein C-terminus binding; ephrin receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; insulin receptor binding; protein kinase activity; integrin binding; protein binding; enzyme binding; SH3/SH2 adaptor activity; protein kinase C binding; protein-tyrosine kinase activity; heme binding; estrogen receptor binding; kinase activity; SH2 domain binding; ATP binding; receptor binding; hormone receptor binding

Biological Process: oogenesis; regulation of estrogen receptor signaling pathway; central nervous system development; estrogen receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; progesterone receptor signaling pathway; nerve growth factor receptor signaling pathway; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; uterus development; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; positive regulation of MAP kinase activity; cell-cell adhesion; transforming growth factor beta receptor signaling pathway; response to electrical stimulus; cell adhesion; bone resorption; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; activation of protein kinase B; response to virus; transcytosis; positive regulation of integrin activation; positive regulation of protein amino acid autophosphorylation; cellular response to insulin stimulus; response to mechanical stimulus; T cell costimulation; regulation of vascular permeability; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of apoptosis; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; negative regulation of caspase activity; positive regulation of smooth muscle cell migration; signal transduction; regulation of cell-cell adhesion; forebrain development; ephrin receptor signaling pathway; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; response to nutrient levels; regulation of bone resorption; negative regulation of focal adhesion formation; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to mineralocorticoid stimulus; cell cycle; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; cell proliferation; peptidyl-serine phosphorylation; response to hydrogen peroxide; regulation of protein binding; Ras protein signal transduction; stress fiber formation; innate immune response; positive regulation of insulin receptor signaling pathway; response to acidity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cytokine secretion