Rabbit anti-Human Tau Monoclonal Antibody | anti-Tau antibody

Tau Antibody (pSer202/ pThr205), Clone AH36: Biotin

Cat. Num. AAA8005577

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• Reactivity: Human
• Applications: Western Blot, Dot Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Affinity Purified
• Synonyms: Tau; Monoclonal Antibody; Tau Antibody (pSer202/ pThr205); Clone AH36: Biotin; Rabbit Anti-Human Tau Monoclonal IgG; Tau Antibody (pSer202/ pThr205): Biotin; Tau Antibody; Neurofibrillary tangle protein Antibody; MAPTL Antibody; Microtubule-associated protein tau Antibody; MTBT1 Antibody; Paired helical filament-tau Antibody; TAU Antibody; PHF-tau Antibody; MAPT Antibody; AI413597 antibody; AW045860 antibody; DDPAC antibody; FLJ31424 antibody; FTDP 17 antibody; G protein beta1/gamma2 subunit interacting factor 1 antibody; MAPT antibody; MAPTL antibody; MGC134287 antibody; MGC138549 antibody; MGC156663 antibody; Microtubule associated protein tau antibody; Microtubule associated protein tau isoform 4 antibody; Microtubule-associated protein tau antibody; MSTD antibody; Mtapt antibody; MTBT1 antibody; MTBT2 antibody; Neurofibrillary tangle protein antibody; Paired helical filament tau antibody; Paired helical filament-tau antibody; PHF tau antibody; PHF-tau antibody; PPND antibody; PPP1R103 antibody; Protein phosphatase 1; regulatory subunit 103 antibody; pTau antibody; RNPTAU antibody; TAU antibody; TAU_HUMAN antibody; Tauopathy and respiratory failure; included antibody; anti-Tau antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: AH36
• Specificity: Detects ~79 kDa.
• Purity/Purification: Affinity Purified
• Form/Format: PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 776

• Applicable Applications for anti-Tau antibody: Western Blot (WB), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500; ICC/IF: 1:500
• Immunogen Species: Human
• Immunogen: Synthetic peptide of Human Phospho Tau (Ser202/Thr205)
• Conjugate: Biotin
• Cellular Localization: Cytoskeleton; Cytosol; Plasma Membrane; Axon; Dendrite
• Tissue Specificity: Expressed in neurons.
• Antibody Type: Recombinant Monoclonal
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)

Western Blot (WB)

(Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. pSer202/pThr205 was detected. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample. Courtesy of: Francesco Paonessa.)

Dot Blot (DB)

(Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.)

Related Product Information for anti-Tau antibody

Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3).

Product Categories/Family for anti-Tau antibody

Alzheimer's Disease; Axon Markers; Cell Markers; Cell Signaling; Cytoskeleton; Microtubules; MT Associated Proteins; Neurodegeneration; Neuron Markers; Neuroscience; Tangles & Tau; Antibodies

References

1. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146-148 (1907). 2. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.

NCBI and Uniprot Product Information

• NCBI GI #: 294862258
• NCBI GeneID: 4137
• NCBI Accession #: NP_001116538.2
• NCBI GenBank Nucleotide #: NP_001116538.2
• UniProt Accession #: P10636
• NCBI Official Full Name: microtubule-associated protein tau isoform 6
• NCBI Official Synonym Full Names: microtubule associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Tautomerase
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU; PHF-tau
• UniProt Entry Name: TAU_HUMAN

NCBI Description

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: microtubule; microtubule associated complex; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; enzyme binding; structural constituent of cytoskeleton; microtubule binding; apolipoprotein binding; protein kinase binding; SH3 domain binding

Biological Process: axon extension; apoptosis; positive regulation of microtubule polymerization; positive regulation of axon extension; axon cargo transport; neuron migration; microtubule cytoskeleton organization and biogenesis; adult walking behavior; regulation of microtubule polymerization; mitochondrion transport along microtubule; negative regulation of intracellular transport; generation of neurons; regulation of autophagy; cell structure disassembly during apoptosis

Disease: Supranuclear Palsy, Progressive, 1; Pick Disease Of Brain; Frontotemporal Dementia; Parkinson-dementia Syndrome; Parkinson Disease, Late-onset; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related