Mouse anti-Human FCGR1A / CD64 Monoclonal Antibody | anti-FCGR1A / CD64 antibody

Anti-FCGR1A / CD64 Antibody (clone 3D3) IHC-plus

Cat. Num. AAA249232

• Gene Names: FCGR1A; CD64; FCRI; CD64A; IGFR1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Protein A/G purified
• Synonyms: FCGR1A / CD64; Monoclonal Antibody; Anti-FCGR1A / CD64 Antibody (clone 3D3) IHC-plus; Mouse Monoclonal [clone 3D3] (IgG1) to Human FCGR1A / CD64; Human FCGR1A / CD64; CD64A; CD64 antigen; FCG1; FcgammaRIa; IGFR1; IgG Fc receptor I; Fc-gamma receptor I A1; Fc-gamma RIA; FCGR1; Fc gamma receptor; Fc-gamma RI; FCRI; anti-FCGR1A / CD64 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3D3
• Specificity: Human CD64
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 374

• Applicable Applications for anti-FCGR1A / CD64 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IHC-Paraffin (10 ug/ml); Immunofluorescence (1:100); Western Blot (1:4000); (optimal dilution to be determined by the researcher)
• Immunogen Species: FCGR1A / CD64 antibody was raised against Human
• Immunogen: FCGR1A / CD64 antibody was raised against full length human recombinant protein of human FCGR1A(NP_000557) produced in HEK293T cell.
• Antigen Type: Recombinant protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles. Stable for 12 months from date of receipt if properly stored.

NCBI and Uniprot Product Information

• NCBI GI #: 10835133
• NCBI GeneID: 2209
• NCBI Accession #: NP_000557.1
• NCBI GenBank Nucleotide #: NM_000566.3
• UniProt Accession #: P12314; P12315; Q5QNW7; Q92495; Q92663
• NCBI Official Full Name: high affinity immunoglobulin gamma Fc receptor I
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor Ia
• NCBI Official Symbol: FCGR1A
• NCBI Official Synonym Symbols: CD64; FCRI; CD64A; IGFR1
• NCBI Protein Information: high affinity immunoglobulin gamma Fc receptor I
• UniProt Protein Name: High affinity immunoglobulin gamma Fc receptor I
• UniProt Gene Name: FCGR1A
• UniProt Synonym Gene Names: FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
• UniProt Entry Name: FCGR1_HUMAN

NCBI Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: early endosome membrane; integral to membrane; plasma membrane

Molecular Function: IgG binding; protein binding; receptor signaling protein activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; immune response; innate immune response; phagocytosis, engulfment; regulation of immune response; signal transduction