Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. MBS9200249 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Mouse Beta-Actin Monoclonal Antibody | anti-ACTB antibody
Beta-Actin Antibody
IHC~~1:10~50
IF~~1:10~50
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. MBS9200249 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. MBS9200249 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(ACTB Antibody (MBS9200249)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ACTB Antibody (MBS9200249)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(Western blot analysis of anti-ACTB Antibody in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.)
Western Blot (WB)
(Western blot analysis of anti-ACTB Antibody in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
Uniprot Description
ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 7p22
Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; focal adhesion; protein complex; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane
Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; Tat protein binding; kinesin binding; nitric-oxide synthase binding; protein kinase binding; ATP binding
Biological Process: intercellular junction assembly and maintenance; axon guidance; establishment and/or maintenance of chromatin architecture; protein folding; 'de novo' posttranslational protein folding; retinal homeostasis; substantia nigra development; cellular protein metabolic process; ATP-dependent chromatin remodeling; ephrin receptor signaling pathway; innate immune response; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway
Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1
Research Articles on ACTB
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Product Notes
The ACTB actb (Catalog #AAA9200249) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Beta-Actin Antibody reacts with Human, mouse (Predicted Reactivity: Xenopus, Bovine, Chicken, Monkey, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~1:1000 IHC~~1:10~50 IF~~1:10~50. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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