anti-Human AXL Monoclonal Antibody | anti-AXL antibody

Mouse Monoclonal [clone 7E10] (IgG1) to Human AXL

Cat. Num. AAA245668

• Gene Names: AXL; ARK; UFO; JTK11; Tyro7
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Ascites
• Synonyms: AXL; Monoclonal Antibody; Mouse Monoclonal [clone 7E10] (IgG1) to Human AXL; Anti-AXL Antibody (clone 7E10) IHC-plus; AXL transforming sequence/gene; Oncogene AXL; AXL oncogene; AXL receptor tyrosine kinase; JTK11; Tyro7; UFO; Human AXL; anti-AXL antibody

• Host: AXL antibody was produced in Mouse.
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 70000000000
• Specificity: Human
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide
• Sequence Length: 885

• Applicable Applications for anti-AXL antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry-Paraffin (IHC-P) 1:200; Immunofluorescence (IF) 1:200-1:1000; Western Blot (WB) 1:100 - 1:500; ELISA (EIA) 1:10000; ; Optimal dilution to be determined by the researcher.
• Target Species: Human
• Immunogen Species: AXL antibody was raised against Human.
• Immunogen: AXL antibody was raised against purified recombinant extracellular fragment of human AXL fused with hIgGFc tag expressed in HEK293 cell line.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Short term: 4°C; Long term: -20°C.

Immunohistochemistry (IHC)

(Anti-AXL antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-AXL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Western Blot (WB)

(Confocal immunofluorescence of methanol-fixed HEK293 cells transfected with AXL-hIgGFc using anti-AXL monoclonal antibody(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.)

Western Blot (WB)

(Western blot of anti-AXL monoclonal antibody against extracellular domain of human AXL(aa19-444).)

Related Product Information for anti-AXL antibody

Axl, an Axl type tyrosine kinase, is characterized by an extracellular domain resembling cell adhesion molecules and an intracellular conserved tyrosine kinase domain. Axl has been reported to induce cell proliferation and transformation, and is important in the vasculature functions. Expression of AXL has been seen in many cancers, including those of the lung, breast, stomach, thyroid, liver, and more. Two protein isoforms of Axl have been documented.

Product Categories/Family for anti-AXL antibody

Family: Protein Kinase; Subfamily: Axl

NCBI and Uniprot Product Information

• NCBI GI #: 21536468
• NCBI GeneID: 558
• NCBI Accession #: NP_001690.2
• NCBI GenBank Nucleotide #: NM_001699.5
• UniProt Accession #: P30530; Q8N5L2; Q9UD27
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 2
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: ARK; UFO; JTK11; Tyro7
• NCBI Protein Information: tyrosine-protein kinase receptor UFO; AXL oncogene; AXL transforming sequence/gene
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Kinase, protein; Oncoprotein; TK group; Axl family

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding

Biological Process: peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; signal transduction; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; negative regulation of neuron apoptosis; inflammatory response; secretion by cell; forebrain cell migration; platelet activation; negative regulation of lymphocyte activation; organ regeneration; vagina development; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; blood vessel remodeling; spermatogenesis; positive regulation of cytokine and chemokine mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; apoptotic cell clearance