Mouse anti-Mouse Qa-1b Monoclonal Antibody

Qa-1b (H2 T23, H2 Class I Histocompatibility Antigen D37 alpha Chain, H2 Q1, Histocompatibility 2 T Region Locus 23, MHC Class I Antigen Qa1, MHC ClassIb Qa1, OTTMUSP00000015895, Qa1)

Cat. Num. AAA6007408

• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Neutralization Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Qa-1b; Monoclonal Antibody; Qa-1b (H2 T23; H2 Class I Histocompatibility Antigen D37 alpha Chain; H2 Q1; Histocompatibility 2 T Region Locus 23; MHC Class I Antigen Qa1; MHC ClassIb Qa1; OTTMUSP00000015895; Qa1); Anti -Qa-1b (H2 T23; anti-Qa-1b antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 11E331
• Specificity: Recognizes mouse Qa-1b.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-Qa-1b antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Neutralization
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Flow Cytometry.; Dilution: Flow Cytometry: 0.1-0.5ug/10e6 cells; Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to the alpha-2 domain of Qa-1b.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Qa-1b in mouse thymus tissue lysate using MBS6007408 at 2ug/ml.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Qa-1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) MBS6007408 (with an anti-mouse IgG1-PE secondary) at 0.25ug/10e6 cells. A shift is seen in the entire population of cells.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Qa-1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) MBS6007408 (with an anti-mouse IgG1-PE secondary) at 0.25ug/10e6 cells. A shift is seen in only a small percentage of cells.)

Related Product Information for anti-Qa-1b antibody

Qa-1, a nonclassical class I histocompatibility molecule encoded by the T23 gene in the T region expressed in mice, predominantly assembles with a single nonameric peptide, Qdm, derived from the signal sequence of certain class Ia molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) express on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1, expressing on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1 serves for MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1 is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. The 6A8.6F10.1A6 mAb can be used for flow cytometric assay to detect Qa-1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa-1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa-1 restricted antigen presentation and other functional study where Qa-1b associated immuno-regulation may work.

Product Categories/Family for anti-Qa-1b antibody

Antibodies; Abs to HLA