Western Blot (WB)
(Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
Mouse anti-Human SOX2 Monoclonal Antibody | anti-SOX2 antibody
SOX2 Antibody
IF~~1:100
IHC-P~~1:50~100
FC~~1:10~50
Western Blot (WB)
(Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
Western Blot (WB)
(Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
Western Blot (WB)
(Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody. AM2048a was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
Western Blot (WB)
(Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody. AM2048a was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20?g.)
Western Blot (WB)
(Western blot analysis of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.)
Western Blot (WB)
(Western blot analysis of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.)
Western Blot (WB)
(Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody. 293 cell lysates (2 ?g/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies))
Western Blot (WB)
(Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody. 293 cell lysates (2 ?g/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies))
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C. SOX2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C. SOX2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.)
1.Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095.
2.Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605.
3.Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983.
4.Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911.
4.Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695.
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
References for MCF7 cell line:
1.Soule, HD; Vazquez J; Long A; Albert S; Brennan M. (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute 51 (5): 1409-1416. [PMID 4357757].
2.Levenson, AS; Jordan VC. (1997). "MCF-7: the first hormone-responsive breast cancer cell line". Cancer Research 57 (15): 3071-3078. [PMID 9242427].
3.Lacroix, M; Leclercq G. (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Research and Treatment 83 (3): 249-289.[PMID 14758095].
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Uniprot Description
SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.
Protein type: Transcription factor; DNA-binding; Cell development/differentiation
Chromosomal Location of Human Ortholog: 3q26.3-q27
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; miRNA binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; chromatin binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; tongue development; establishment and/or maintenance of chromatin architecture; somatic stem cell maintenance; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; diencephalon morphogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; olfactory placode formation; forebrain neuron differentiation; detection of mechanical stimulus involved in equilibrioception; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; tissue regeneration; forebrain development; male genitalia development; response to wounding; positive regulation of cell-cell adhesion; positive regulation of epithelial cell differentiation; adenohypophysis development; negative regulation of osteoblast differentiation; cell cycle arrest; inner ear development; positive regulation of Notch signaling pathway; negative regulation of epithelial cell proliferation; positive regulation of neuroblast proliferation; inner ear morphogenesis; response to retinoic acid; neuron fate commitment; detection of mechanical stimulus involved in sensory perception of sound; osteoblast differentiation; negative regulation of neuron differentiation; eye development; pituitary gland development; regulation of gene expression; glial cell fate commitment; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; positive regulation of neuron differentiation; pigment biosynthetic process; alveolus development; retina morphogenesis in camera-type eye
Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia
Research Articles on SOX2
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Product Notes
The SOX2 sox2 (Catalog #AAA9200597) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SOX2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry - Paraffin (IHC-P), Flow Cytometry (FC/FACS), ELISA. WB~~1:200~2000 IF~~1:100 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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