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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Immunity" - showing 500-550
Western Blot (WB)
(Western blot analysis of COLEC11 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). COLEC11 (arrow) was detected using the purified Pab.)
COLEC11, Polyclonal Antibody (Cat# AAA9214722)
Full Name
COLEC11 Antibody (N-term)
Gene Names
COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.Result: MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.)
Interleukin 6 (IL6), Active Protein (Cat# AAA2097342)
Full Name
Active Interleukin 6 (IL6)
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Activity Data
(Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Growth Regulated Oncogene Beta (GROb), Active Protein (Cat# AAA2097295)
Full Name
Active Growth Regulated Oncogene Beta (GROb)
Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Activity Data
(Figure. The binding activity of IGF2 with EMMPRIN.Insulin-like growth factor 2 (IGF2) is one of three protein hormones that share structural similarity to insulin. It has growth-regulating, insulin-like and mitogenic activities. IGF2 exerts its effects by binding to the IGF-1 receptor and to the short isoform of the insulin receptor. IGF2 may also bind to the IGF2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. Besides, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) has been identified as an interactor of IGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGF2 and recombinant human EMMPRIN. Briefly, IGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EMMPRIN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IGF2 and EMMPRIN was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin Like Growth Factor 2 (IGF2), Active Protein (Cat# AAA2105064)
Full Name
Active Insulin Like Growth Factor 2 (IGF2)
Gene Names
IGF2; GRDF; IGF-II; PP9974; C11orf43
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$160/0.01 mg | $290/0.05 mg | $445/0.1 mg | $540/0.2 mg | $1,045/0.5 mg | $1,535/1 mg | $3,765/5 mg
Activity Data
(Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner.)
Suppressors Of Cytokine Signaling 3 (SOCS3), Active Protein (Cat# AAA2097298)
Full Name
Active Suppressors Of Cytokine Signaling 3 (SOCS3)
Gene Names
Socs3; Cis3; Ef10; Ssi3; Cish3; EF-10; SSI-3
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Immunohistochemistry (IHC)
CD163, Polyclonal Antibody (Cat# AAA540877)
Full Name
CD163 Antibody
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Monkey
Applications
EIA, IHC, IF, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Secretory Component Rabbit Recombinant Monoclonal (ECM1/2889R).)
Secretory Component/ECM1, Monoclonal Antibody (Cat# AAA4380544)
Full Name
Secretory Component/ECM1
Gene Names
ECM1; URBWD
Reactivity
Human, Rat. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488349)
Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339ug/mL.)
Vascular Endothelial Growth Factor C (VEGFC), Active Protein (Cat# AAA2097267)
Full Name
Active Vascular Endothelial Growth Factor C (VEGFC)
Gene Names
VEGFC; VRP; Flt4-L; LMPH1D
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA2105151)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$160/0.01 mg | $285/0.05 mg | $440/0.1 mg | $540/0.2 mg | $1,035/0.5 mg | $1,525/1 mg | $3,730/5 mg
Sequence Information
Immunoglobulin A (IgA), Small Molecule (Cat# AAA2097383)
Full Name
OVA Conjugated Immunoglobulin A (IgA)
Gene Names
Igh-VJ558; AI893585
Reactivity
Mouse
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Activity Data
(NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.)
Neuraminidase (NEU), Active Protein (Cat# AAA2097248)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Activity Data
(Figure. The binding activity of IGFBP2 with IGFBP2.Insulin Like Growth Factor Binding Protein 2 (IGFBP2) is a menber of IGF binding proteins (IGFBPs) family. IGF binding proteins (IGFBPs) are proteins of 24 to 45kDa. Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Besides, Insulin Like Growth Factor 2 (IGF2) has been identified as an interactor of IGFBP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGFBP2 and recombinant mouse IGF2. Briefly, IGFBP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IGF2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGFBP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IGFBP2 and IGFBP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin Like Growth Factor Binding Protein 2 (IGFBP2), Active Protein (Cat# AAA2105065)
Full Name
Active Insulin Like Growth Factor Binding Protein 2 (IGFBP2)
Gene Names
Igfbp2; IBP-2; Igfbp-2; AI255832; mIGFBP-2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Sequence Information
Trefoil Factor 3, Intestinal (TFF3), Small Molecule (Cat# AAA2097399)
Full Name
OVA Conjugated Trefoil Factor 3, Intestinal (TFF3)
Gene Names
Tff3; ITF
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $805/0.5 mg | $1,180/1 mg | $2,885/5 mg | $12,970/5x5 mg
Activity Data
(Figure1. TRAP levels in the cell of RAW264.7 cells induced by RANKL.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. The protein can bind to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. Thus, we use recombinant RANKL to induce RAW264.7 mouse monocyte/macrophage cells to differentiate to osteoclast cell. RAW264.7 mouse monocyte/macrophage cells were seeded into 24-well plates at a density of 2×105 and allowed to attach overnight, then treated with RANKL (1ng/mL, 10ng/mL) and incubated for 72h. Then we assay TRAP (a kind of enzyme expressed by osteoclast cell) by ELASA. Result: TRAP levels in the cell supernatant of RAW264.7 cells increased significantly after stimulated with RANK, the data was shown in Table 1 and Figure1.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105123)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (MBS1750500).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD. )
PF4/Cxcl4, Polyclonal Antibody (Cat# AAA1750500)
Full Name
Anti-PF4/Cxcl4 Picoband antibody
Gene Names
Pf4; PF-4; Pf4a; Cxcl4; RATPF4A
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10uL of CCK-8 solution was added to each well of the plate, the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope was shown in Figure 1.)
Interleukin 2 (IL2), Active Protein (Cat# AAA2097325)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Activity Data
(Figure. The binding activity of BDNF with APP.Brain-derived neurotrophic factor, also known as BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat BDNF and recombinant rat APP. Briefly, BDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of BDNF and APP was shown in Figure 1, and this effect was in a dose dependent manner.)
Brain Derived Neurotrophic Factor (BDNF), Active Protein (Cat# AAA2105048)
Full Name
Active Brain Derived Neurotrophic Factor (BDNF)
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Activity Data
(Figure. The binding activity of S100A8 with S100A9.S100 calcium-binding protein A8 (S100A8) also known as calgranulin A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Besides, S100 Calcium Binding Protein A9 (S100A9) has been identified as an interactor of S100A8, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100A8 and recombinant rat S100A9. Briefly, S100A8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to S100A9-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of S100A8 and S100A9 was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA2105152)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100a8; Mrp8
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $645/0.2 mg | $1,250/0.5 mg | $1,860/1 mg | $4,570/5 mg
Immunohistochemistry (IHC)
(Fig-2: Immunohistochemical analysis of TLR2 in human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
TLR2, Monoclonal Antibody (Cat# AAA668966)
Full Name
NALE Monoclonal Antibody to TLR2 (Clone: ABM3A87) (No Azide Low Endotoxin)
Gene Names
TLR2; TIL4; CD282
Reactivity
Mouse, Human
Applications
IHC, WB, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Activity Data
(The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner.)
Coagulation Factor VII (F7), Active Protein (Cat# AAA2097300)
Full Name
Active Coagulation Factor VII (F7)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$270/0.01 mg | $600/0.05 mg | $930/0.1 mg | $1,145/0.2 mg | $2,260/0.5 mg | $3,350/1 mg | $8,205/5 mg
Activity Data
(Figure. The binding activity of IL8 with SDC1.Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cellsendothelial cells. IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. IL8 is also known to be a potent promoter of angiogenesis. Besides, Syndecan 1 (SDC1) has been identified as an interactor of IL8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL8 and recombinant human SDC1. Briefly, IL8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SDC1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL8 and SDC1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 8 (IL8), Active Protein (Cat# AAA2105072)
Full Name
Active Interleukin 8 (IL8)
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Activity Data
(Figure. The binding activity of ODC with TK1.The enzyme ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. This reaction is the committed step in polyamine synthesis. Lysine 69 on ornithine decarboxylase (ODC) binds the cofactor pyridoxal phosphate to form a Schiff base. Ornithine displaces the lysine to form a Schiff base attached to ODC, which decarboxylates to form a quinoid intermediate. This intermediate rearranges to form a Schiff base attached to putrescine, which is attacked by lysine to release putrescine product and reform PLP-bound ODC. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ODC and recombinant rat TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105138)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
Odc1; Odc
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,020/0.2 mg | $1,985/0.5 mg | $2,945/1 mg | $7,200/5 mg
Activity Data
((FGF1) Fibroblast growth factor 1 belongs to the fibroblast growth factor (FGF) family. FGF1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 1, Acidic (FGF1), Active Protein (Cat# AAA2097255)
Full Name
Active Fibroblast Growth Factor 1, Acidic (FGF1)
Gene Names
Fgf1; Fam; Fgfa; Dffrx; Fgf-1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$130/0.01 mg | $220/0.05 mg | $305/0.1 mg | $375/0.2 mg | $705/0.5 mg | $1,045/1 mg | $2,540/5 mg | $11,420/5x5 mg
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
B7-H4, Monoclonal Antibody (Cat# AAA4381352)
Full Name
B7-H4 (Immuno-Inhibitory Protein)
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Human
Applications
EIA, FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA9211301)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 (MBS488444) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488444, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488444)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galiximab) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD80, Monoclonal Recombinant Antibody (Cat# AAA488348)
Full Name
Anti-CD80 [IDEC-114 (Galiximab)]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, FC/FACS, WB, EIA, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein (Cat# AAA2097282)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Applications
Cell culture, Activity Assays
Purity
>85%
Pricing
$250/0.01 mg | $550/0.05 mg | $855/0.1 mg | $1,055/0.2 mg | $2,075/0.5 mg | $3,075/1 mg | $7,525/5 mg
Sequence
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA2105091)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>95%
Pricing
$250/0.01 mg | $555/0.05 mg | $865/0.1 mg | $1,065/0.2 mg | $2,095/0.5 mg | $3,100/1 mg | $7,590/5 mg
Activity Data
(Figure. The binding activity of DKK1 with CTNNb1.)
Dickkopf Related Protein 1 (DKK1), Active Protein (Cat# AAA2097279)
Full Name
Active Dickkopf Related Protein 1 (DKK1)
Gene Names
DKK1; SK; DKK-1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $500/0.05 mg | $775/0.1 mg | $960/0.2 mg | $1,870/0.5 mg | $2,770/1 mg | $6,775/5 mg
Activity Data
(Figure. The binding activity of IAP with THBS1.)
Integrin Associated Protein (IAP), Active Protein (Cat# AAA2097286)
Full Name
Active Integrin Associated Protein (IAP)
Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Western Blot (WB)
(Western blot analysis of BRI3 using Jurkat whole cell lysates)
BRI3, Polyclonal Antibody (Cat# AAA9609601)
Full Name
BRI3 Antibody
Gene Names
BRI3; I3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGF121. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. The result was shown in Figure 2. It was obvious that VEGF121 significantly increased cell viability of ECV-304 cells.)
Vascular Endothelial Growth Factor 121 (VEGF121), Active Protein (Cat# AAA2097243)
Full Name
Active Vascular Endothelial Growth Factor 121 (VEGF121)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Activity Data
(Figure. The binding activity of S100B with RAGE.Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2105105)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Immunofluorescence (IF)
(MBS9605555 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GIMAP2, Polyclonal Antibody (Cat# AAA9605555)
Full Name
GIMAP2 Antibody
Gene Names
GIMAP2; IAN12; IMAP2; HIMAP2
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Sequence
Carcinoembryonic Antigen (CEA), Active Protein (Cat# AAA2105085)
Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Cell Culture, Activity Assays
Purity
>95%
Pricing
$195/0.01 mg | $395/0.05 mg | $610/0.1 mg | $750/0.2 mg | $1,455/0.5 mg | $2,165/1 mg | $5,280/5 mg
Western Blot (WB)
(Western blot analysis IRGC using HeLa whole cell lysates)
IRGC, Polyclonal Antibody (Cat# AAA9609295)
Full Name
IRGC Antibody
Gene Names
IRGC; IFGGE; IRGC1; Iigp5; CINEMA; R30953_1
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Factor Related Apoptosis (FAS), Active Protein (Cat# AAA2097254)
Full Name
Active Factor Related Apoptosis (FAS)
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$250/0.01 mg | $545/0.05 mg | $845/0.1 mg | $1,050/0.2 mg | $2,050/0.5 mg | $3,040/1 mg | $7,440/5 mg
Activity Data
(Figure. The binding activity of Slit2 with GREM1.Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Slit Homolog 2 (Slit2), Active Protein (Cat# AAA2105109)
Full Name
Active Slit Homolog 2 (Slit2)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $515/0.05 mg | $795/0.1 mg | $985/0.2 mg | $1,920/0.5 mg | $2,845/1 mg | $6,960/5 mg
Sequence Information
Cyclophilin A (CYPA), Small Molecule (Cat# AAA2097386)
Full Name
OVA Conjugated Cyclophilin A (CYPA)
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $340/0.1 mg | $420/0.2 mg | $795/0.5 mg | $1,165/1 mg | $2,840/5 mg | $12,775/5x5 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000267)
Full Name
Cyclooxygenase 1 Antibody: ATTO 488
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Activity Data
(TNFRSF14 (Tumor necrosis factor receptor superfamily member 14) belongs to the tumor necrosis factor receptor superfamily. TNFRSF14 functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. A binding ELISA assay was conducted to detect the association of TNFRSF14 with TNFa. Briefly, TNFRSF14 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF14 were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF14 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF14 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14), Active Protein (Cat# AAA2097313)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)
Gene Names
TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097340)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Activity Data
(NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.)
Neuraminidase (NEU), Active Protein (Cat# AAA2097247)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Activity Data
(Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.)
Interleukin 6 (IL6), Active Protein (Cat# AAA2097327)
Full Name
Active Interleukin 6 (IL6)
Gene Names
IL6; IL-6; CHIL-6; interleukin-6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Activity Data
(Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. Thus we have chosed casein-zymography to measure the activity of MMP13. Briefly, various concentrations of MMP13 (10ug, 5ug, 1ug, 0.1ug, 0.01ug) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and trypsase (1ug/mL) as positive control.Result : Casein hydrolysis by recombinant human MMP13 was shown in figure 1.)
Matrix Metalloproteinase 13 (MMP13), Active Protein (Cat# AAA2105075)
Full Name
Active Matrix Metalloproteinase 13 (MMP13)
Gene Names
MMP13; CLG3; MDST; MANDP1; MMP-13
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of CTLA4 using anti-CTLA4 antibody (MBS1750305).
CTLA4 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (MBS1750305) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
CTLA4, Polyclonal Antibody (Cat# AAA1750305)
Full Name
Anti-CTLA4 Picoband Antibody
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(The anti-HK1 Pab is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA9204225)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(MBS9605556 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
GIMAP4, Polyclonal Antibody (Cat# AAA9605556)
Full Name
GIMAP4 Antibody
Gene Names
GIMAP4; IAN1; IAN-1; IMAP4; MSTP062
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
