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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Immunity" - showing 600-650
Western Blot (WB)
(Western Blot Analysis of human HeLa and MCF-7 cell lysate with ENAH/MENA Mouse Monoclonal Antibody (ENAH/1988).)
ENAH/MENA, Monoclonal Antibody (Cat# AAA4381196)
Full Name
ENAH/MENA (Actin Regulator)
Gene Names
ENAH; ENA; MENA; NDPP1
Reactivity
Human
Applications
EIA, WB
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Sequence Information
Y-Box Binding Protein 1 (YBX1), Recombinant Protein (Cat# AAA2097535)
Full Name
Recombinant Y-Box Binding Protein 1 (YBX1)
Gene Names
YBX1; YB1; NSEP1
Reactivity
Human
Applications
WB
Purity
> 95%
Pricing
$130/0.01 mg | $215/0.05 mg | $295/0.1 mg | $365/0.2 mg | $685/0.5 mg | $1,010/1 mg | $2,460/5 mg | $11,065/5x5 mg
Western Blot (WB)
TLR10, Polyclonal Antibody (Cat# AAA540461)
Full Name
TLR10 Antibody
Gene Names
TLR10; CD290
Reactivity
Chimpanzee, Human, Monkey, Pig, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488454)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000276)
Full Name
Cyclooxygenase 1 Antibody: Biotin
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000014)
Full Name
IRGM Antibody: ATTO 390
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Sequence
Elastase 2, Neutrophil, Active Protein (Cat# AAA2105086)
Full Name
Active Elastase 2, Neutrophil (ELA2)
Applications
Cell culture, Activity Assays
Purity
> 98%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000029)
Full Name
IRGM Antibody: RPE
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).)
CD73, Monoclonal Antibody (Cat# AAA4381133)
Full Name
CD73 (Immuno-Oncology Target)
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
EIA, FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (MBS1750819).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD. )
Thrombopoietin, Polyclonal Antibody (Cat# AAA1750819)
Full Name
Anti-Thrombopoietin Picoband Antibody
Gene Names
Thpo; Ml; Tpo; Mgdf; Mpllg
Reactivity
Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Fig-1: Immunohistochemical analysis of TLR7 in human Kidney tissue using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)
TLR7, Monoclonal Antibody (Cat# AAA668962)
Full Name
NALE Monoclonal Antibody to TLR7 (Clone: ABM2C27) (No Azide Low Endotoxin)
Gene Names
TLR7; TLR7-like
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Activity Data
(The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL4 and recombinant mouse IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of IL4 with IL2Rg.)
Interleukin 4 (IL4), Active Protein (Cat# AAA2105070)
Full Name
Active Interleukin 4 (IL4)
Gene Names
Il4; Il-4; BSF-1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $400/0.05 mg | $615/0.1 mg | $755/0.2 mg | $1,460/0.5 mg | $2,175/1 mg | $5,305/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (MBS1750778).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: human A549 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD. )
NFATC4, Polyclonal Antibody (Cat# AAA1750778)
Full Name
Anti-NFATC4 Picoband Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
TLR1, Polyclonal Antibody (Cat# AAA540395)
Full Name
TLR1 Antibody
Reactivity
Mouse, Rat
Predicted: Human
Predicted: Human
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD81 antibody 1D6 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 (MBS488457) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488457, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
CD81, Monoclonal Recombinant Antibody (Cat# AAA488457)
Full Name
Anti-CD81 [1D6]
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Goat, Sheep
Applications
EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809872)
Full Name
PI 3 Kinase Class 3 antibody: ATTO 594
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Activity Data
(Figure. The binding activity of IL28B with IL10Rb.Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 28B (IL28B), Active Protein (Cat# AAA2105159)
Full Name
Active Interleukin 28B (IL28B)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Activity Data
(Figure. The binding activity of IL33 with IL1RL1.Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 33 (IL33), Active Protein (Cat# AAA2105157)
Full Name
Active Interleukin 33 (IL33)
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,010/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IL12A using anti-IL12A antibody (MBS1750514).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysates,Lane 2: human SW620 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12A antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL12A at approximately 35KD. The expected band size for IL12A is at 25KD. )
IL12A/Il 12, Polyclonal Antibody (Cat# AAA1750514)
Full Name
Anti-IL12A/Il 12 Picoband antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(Figure. Cell proliferation of A549 cells after stimulated with GREM1.Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant mouse GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly,10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GREM1 for 96h. The result was shown in Figure 2. It was obvious that GREM1 significantly increased cell viability of A549 cells.(A) A549 cells cultured in DMEM, stimulated with 1ng/mL GREM1 for 96h; (B) Unstimulated A549 cells cultured in DMEM for 96h.)
Gremlin 1 (GREM1), Active Protein (Cat# AAA2105166)
Full Name
Active Gremlin 1 (GREM1)
Gene Names
Grem1; ld; Drm; Grem; Cktsf1b1
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$205/0.01 mg | $405/0.05 mg | $625/0.1 mg | $770/0.2 mg | $1,495/0.5 mg | $2,225/1 mg | $5,435/5 mg
Western Blot (WB)
(Western Blot: Sample: Recombinant TFR, Human.)
Transferrin Receptor (TFR), Polyclonal Antibody (Cat# AAA2034677)
Full Name
Anti-Transferrin Receptor (TFR) polyclonal Antibody
Gene Names
TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
Reactivity
Human
Applications
WB
Purity
Antigen-specific affinity chromatography
Pricing
$155/0.1 mL | $195/0.2 mL | $315/0.5 mL | $390/1 mL | $1,130/5x1 mL
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant FceRI, Mouse)
Receptor I For The Fc Region Of Immunoglobulin E (FceRI), Polyclonal Antibody (Cat# AAA2111389)
Full Name
Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin E (FceRI)
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Activity Data
(Figure 1. The chemotactic effect of SDF1 on THP1 cells.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 10ng/ml SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h. )
Stromal Cell Derived Factor 1 (SDF1), Active Protein (Cat# AAA2097351)
Full Name
Active Stromal Cell Derived Factor 1 (SDF1)
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $595/0.2 mg | $1,145/0.5 mg | $1,695/1 mg | $4,160/5 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000269)
Full Name
Cyclooxygenase 1 Antibody: ATTO 594
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Testing Data
(Binding curve of anti-IgE antibody TES-C21 to human IgE Fc domain ELISA Plate coated with human IgE Fc domain (Pr00104-14.5) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
IgE, Monoclonal Recombinant Antibody (Cat# AAA488431)
Full Name
Anti-IgE [TES-C21]
Reactivity
Human
Applications
BL, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809880)
Full Name
PI 3 Kinase Class 3 antibody: FITC
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809967)
Full Name
RIP3 Antibody: Alkaline Phosphatase
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Crp using anti-Crp antibody (MBS1750357).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crp antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Crp at approximately 25KD. The expected band size for Crp is at 25KD. )
Crp/C Reactive Protein, Polyclonal Antibody (Cat# AAA1750357)
Full Name
Anti-Crp/C Reactive Protein Picoband Antibody
Gene Names
Crp; AI255847
Reactivity
Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SCF using anti-SCF antibody (MBS1750603).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCF at approximately 37KD. The expected band size for SCF is at 31KD. )
SCF, Polyclonal Antibody (Cat# AAA1750603)
Full Name
Anti-SCF Picoband Antibody
Gene Names
KITLG; SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Sequence Information
Major Basic Protein (MBP), Small Molecule (Cat# AAA2097395)
Full Name
OVA Conjugated Major Basic Protein (MBP)
Gene Names
PRG2; MBP; BMPG; MBP1; proMBP
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Sequence Information
Procalcitonin (PCT), Small Molecule (Cat# AAA2097355)
Full Name
OVA Conjugated Procalcitonin (PCT)
Gene Names
CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Activity Data
(IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.Figure. The binding activity of IL17C with IL17RA.)
Interleukin 17C (IL17C), Active Protein (Cat# AAA2097330)
Full Name
Active Interleukin 17C (IL17C)
Gene Names
IL17C; CX2; IL-17C
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809877)
Full Name
PI 3 Kinase Class 3 antibody: Alkaline Phosphatase
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809973)
Full Name
RIP3 Antibody: PerCP
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Sequence Information
Interleukin 1 Beta (IL1b), Small Molecule (Cat# AAA2097396)
Full Name
OVA Conjugated Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Reactivity
Mouse
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $805/0.5 mg | $1,180/1 mg | $2,885/5 mg | $12,970/5x5 mg
Typical Testing Data/Standard Curve (for reference only)
Liver X Receptor Alpha (LXRa), ELISA Kit (Cat# AAA2020587)
Full Name
Mouse Liver X Receptor Alpha (LXRa) ELISA Kit
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Mouse
Pricing
$540/48-Strip-Wells | $730/96-Strip-Wells | $2,935/5x96-Strip-Wells | $5,345/10x96-Strip-Wells | $350/24-Strip-Wells
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (MBS488455) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488455, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488455)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(Macrophage migration inhibitory factor (MIF), also known as glycosylation- inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG- coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MIF with MHCDG.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105111)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809958)
Full Name
RIP3 Antibody
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.)
IRGC, Polyclonal Antibody (Cat# AAA151122)
Full Name
IRGC Antibody
Gene Names
IRGC; IFGGE; IRGC1; Iigp5; CINEMA; R30953_1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF
Purity
IRGC Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Activity Data
(IL25 (Interleukin-25), also known as IL17E, is reported to induce NF-kappaB activation, and stimulate the production of IL-8. Besides, this cytokine shares the sequence similarity with IL17, which is considered as a ligand for IL17RA. Thus, a binding ELISA assay was conducted to detect the association of recombinant human IL25 with IL17RA. Briefly, IL25 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL25 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL25 with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 25 (IL25), Active Protein (Cat# AAA2097332)
Full Name
Active Interleukin 25 (IL25)
Gene Names
IL25; IL17E
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (MBS1750330).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL33 at approximately 31-37KD. The expected band size for IL33 is at 31KD. )
IL33, Polyclonal Antibody (Cat# AAA1750330)
Full Name
Anti-IL33 Picoband antibody
Gene Names
Il33; RGD1311155
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Binding curve of anti-IgE antibody TES-C21 to human IgE Fc domain ELISA Plate coated with human IgE Fc domain at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
IgE, Monoclonal Recombinant Antibody (Cat# AAA488430)
Full Name
Anti-IgE [TES-C21]
Reactivity
Human
Applications
BL, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(Interleukin-17 receptor A (IL17RA) is a cytokine receptor which binds interleukin 17A (IL17A). IL17A is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA, as a transmembrane protein, is a ubiquitous type I membrane glycoprotein that binds IL17A, IL17F and IL17C. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RA with recombinant human IL17A. Briefly, IL17RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17A-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RA with IL17A was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 17 Receptor A (IL17RA), Active Protein (Cat# AAA2097346)
Full Name
Active Interleukin 17 Receptor A (IL17RA)
Gene Names
IL17RA; CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000266)
Full Name
Cyclooxygenase 1 Antibody: ATTO 390
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000277)
Full Name
Cyclooxygenase 1 Antibody: FITC
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Activity Data
(Figure. The binding activity of IL29 with ATP2B2.Interleukin-29 (IL-29) is a member of the helical cytokine family and is a type III interferon. It is also known as IFNlambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. Besides, ATPase, Ca Transporting, Plasma Membrane 2 (ATP2B2) has been identified as an interactor of IL29, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL29 and recombinant human ATP2B2. Briefly, IL29 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to ATP2B2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL29 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL29 and ATP2B2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 29 (IL29), Active Protein (Cat# AAA2105160)
Full Name
Active Interleukin 29 (IL29)
Gene Names
IFNL1; IL29; IL-29
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000017)
Full Name
IRGM Antibody: ATTO 594
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Sequence Information
Chemokine C-X-C-Motif Receptor 4 (CXCR4), Small Molecule (Cat# AAA2097379)
Full Name
OVA Conjugated Chemokine C-X-C-Motif Receptor 4 (CXCR4)
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $230/0.05 mg | $335/0.1 mg | $415/0.2 mg | $780/0.5 mg | $1,145/1 mg | $2,800/5 mg | $12,580/5x5 mg
Activity Data
(Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner.)
Caspase 1 (CASP1), Active Protein (Cat# AAA2097294)
Full Name
Active Caspase 1 (CASP1)
Gene Names
Casp1; Ice; Il1bc
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $575/0.05 mg | $895/0.1 mg | $1,100/0.2 mg | $2,170/0.5 mg | $3,210/1 mg | $7,865/5 mg
