Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Immunity" - showing 450-500
Western Blot (WB)
(Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.)
PI3 Kinase p85 beta, Monoclonal Antibody (Cat# AAA475225)
Full Name
Anti-PI3 Kinase p85 beta Mouse mAb
Gene Names
PIK3R2; p85; MPPH; P85B; MPPH1; p85-BETA
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Sequence
Endothelial Cell Specific Molecule 1 (ESM1), Active Protein (Cat# AAA2097304)
Full Name
Active Endothelial Cell Specific Molecule 1 (ESM1)
Gene Names
ESM1; endocan
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>92%
Pricing
$225/0.01 mg | $490/0.05 mg | $760/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,630/5 mg
Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), Active Protein (Cat# AAA2097237)
Full Name
Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Pricing
$150/0.01 mg | $270/0.05 mg | $400/0.1 mg | $485/0.2 mg | $935/0.5 mg | $1,375/1 mg | $3,360/5 mg | $15,105/5x5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Plasma Cell Marker Monoclonal Antibody (LIV3G11).)
Plasma Cell Marker, Monoclonal Antibody (Cat# AAA438191)
Full Name
Plasma Cell Marker Mouse Monoclonal Antibody
Reactivity
Human.
Does not react with Rat
Does not react with Rat
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(Figure. The binding activity of VEGF165 with VEGFR1.Vascular endothelial growth factor 165 is one kind of isoforms of Vascular endothelial growth factor A (VEGFA). This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Besides, Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) has been identified as an interactor of VEGF165, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VEGF165 and recombinant human VEGFR1. Briefly, VEGF165 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VEGFR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-VEGF165 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of VEGF165 and VEGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Vascular Endothelial Growth Factor 165 (VEGF165), Active Protein (Cat# AAA2105148)
Full Name
Active Vascular Endothelial Growth Factor 165 (VEGF165)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,005/5 mg
Activity Data
(SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.)
Stromal Cell Derived Factor 1 (SDF1), Active Protein (Cat# AAA2097266)
Full Name
Active Stromal Cell Derived Factor 1 (SDF1)
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
SDS-Page
(SDS-PAGE Analysis Purified BCL-6 Mouse Monoclonal Antibody (BCL6/1718). Confirmation of Integrity and Purity of Antibody.)
CD257/BAFF/TNFSF13B, Monoclonal Antibody (Cat# AAA439850)
Full Name
CD257/BAFF/TNFSF13B
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20
Reactivity
Human. Others not known.
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Western Blot (WB)
CTLA4, Polyclonal Antibody (Cat# AAA540634)
Full Name
CTLA4 Antibody
Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Sequence Information
Interleukin 1 Beta (IL1b), Small Molecule (Cat# AAA2097363)
Full Name
OVA Conjugated Interleukin 1 Beta (IL1b)
Gene Names
Il1b; IL-1F2
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $805/0.5 mg | $1,180/1 mg | $2,885/5 mg | $12,970/5x5 mg
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097288)
Full Name
Active Arginase (ARG)
Gene Names
Arg1; AI; PGIF; Arg-1; AI256583
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $750/0.1 mg | $930/0.2 mg | $1,815/0.5 mg | $2,685/1 mg | $6,570/5 mg
Activity Data
(Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Connective Tissue Growth Factor (CTGF) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FN and recombinant human CTGF. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTGF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of FN and CTGF was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibronectin (FN), Active Protein (Cat# AAA2097258)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$185/0.01 mg | $365/0.05 mg | $560/0.1 mg | $690/0.2 mg | $1,335/0.5 mg | $1,985/1 mg | $4,870/5 mg
Western Blot (WB)
Myeloperoxidase, Polyclonal Antibody (Cat# AAA540041)
Full Name
Myeloperoxidase Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Activity Data
(Platelet Derived Growth Factor Subunit A (PDGFA) is a member of Platelet-derived growth factor (PDGF) family which plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis, i.e. proliferation, of mesenchymal cells such as fibroblasts, osteoblasts, tenocytes, vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. Besides, Platelet Derived Growth Factor Receptor Alpha (PDGFRa) has been identified as an interactor of PDGFA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFA and recombinant human PDGFRa. Briefly, PDGFA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100mul were then transferred to PDGFRa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PDGFA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFA and PDGFRa was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of PDGFA with PDGFRa.)
Platelet Derived Growth Factor Subunit A (PDGFA), Active Protein (Cat# AAA2105097)
Full Name
Active Platelet Derived Growth Factor Subunit A (PDGFA)
Gene Names
PDGFA; PDGF1; PDGF-A
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Sequence
Phospholipase A2, Lipoprotein Associated (LpPLA2), Active Protein (Cat# AAA2105125)
Full Name
Active Phospholipase A2, Lipoprotein Associated (LpPLA2)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Pricing
$210/0.01 mg | $425/0.05 mg | $650/0.1 mg | $805/0.2 mg | $1,565/0.5 mg | $2,330/1 mg | $5,690/5 mg
Sequence Information
Macrophage Inflammatory Protein 1 Alpha (MIP1a), Small Molecule (Cat# AAA2097359)
Full Name
BSA Conjugated Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Gene Names
Ccl3; Scya3; MIP-1a
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Sequence
Interleukin 1 Receptor Antagonist (IL1RA), Active Protein (Cat# AAA2097333)
Full Name
Active Interleukin 1 Receptor Antagonist (IL1RA)
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays, In vivo assays
Purity
>95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Activity Data
(Figure. The binding activity of FGF2 with CASP1.Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-beta is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant bovine FGF2 and recombinant bovine CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibroblast Growth Factor 2 (FGF2), Active Protein (Cat# AAA2105099)
Full Name
Active Fibroblast Growth Factor 2, Basic (FGF2)
Gene Names
FGF2; BFGF; FGF-2; HBGF-2
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Granulocytes, Monoclonal Antibody (Cat# AAA225668)
Full Name
Mouse Anti Pig Granulocytes
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$395/0.1 mg | $1,630/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488443)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with FCGRT Mouse Monoclonal Antibody (FCGRT/2932).)
FCGRT/FcRn, Monoclonal Antibody (Cat# AAA4380899)
Full Name
FCGRT/FcRn (IgG Transporter)
Gene Names
FCGRT; FCRN; alpha-chain
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.)
Interleukin 24 (IL24), Active Protein (Cat# AAA2097224)
Full Name
Active Interleukin 24 (IL24)
Gene Names
IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>92%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Activity Data
(Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C .Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope was shown in Figure 1.)
Epidermal Growth Factor (EGF), Active Protein (Cat# AAA2097344)
Full Name
Active Epidermal Growth Factor (EGF)
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$140/0.01 mg | $230/0.05 mg | $335/0.1 mg | $415/0.2 mg | $780/0.5 mg | $1,145/1 mg | $2,800/5 mg | $12,580/5x5 mg
Activity Data
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
Interleukin 18 (IL18), Active Protein (Cat# AAA2097227)
Full Name
Active Interleukin 18 (IL18)
Gene Names
Il18; Igif; Il-18
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$190/0.01 mg | $380/0.05 mg | $585/0.1 mg | $715/0.2 mg | $1,385/0.5 mg | $2,065/1 mg | $5,070/5 mg
Activity Data
(Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5×106 cells/mL in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF, the data was shown in table 1.)
Lactoferrin (LTF), Active Protein (Cat# AAA2105115)
Full Name
Active Lactoferrin (LTF)
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Applications
WB
Purity
>95%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Figure. The binding activity of CD14 with LBP.Cluster Of Differentiation 14 (CD14), also known as CD14, is a component of the innate immune system. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid. Besides, Lipopolysaccharide Binding Protein (LBP) has been identified as an interactor of CD14, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD14 and recombinant human LBP. Briefly, CD14 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to LBP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD14 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CD14 and LBP was shown in Figure 1, and this effect was in a dose dependent manner.)
Cluster Of Differentiation 14 (CD14), Active Protein (Cat# AAA2105110)
Full Name
Active Cluster Of Differentiation 14 (CD14)
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,015/0.2 mg | $1,985/0.5 mg | $2,935/1 mg | $7,180/5 mg
Activity Data
(S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein A6 (S100A6), Active Protein (Cat# AAA2097347)
Full Name
Active S100 Calcium Binding Protein A6 (S100A6)
Gene Names
S100A6; 2A9; PRA; 5B10; CABP; CACY; S10A6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809969)
Full Name
RIP3 Antibody: Biotin
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Testing Data
(Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1.The binding activity of CTSV with RB1.)
Cathepsin V (CTSV), Active Protein (Cat# AAA2097309)
Full Name
Active Cathepsin V (CTSV)
Gene Names
CTSV; CTSU; CATL2; CTSL2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $930/0.2 mg | $1,820/0.5 mg | $2,690/1 mg | $6,580/5 mg
Sequence Information
Neutrophil Activating Protein 3 (NAP3), Small Molecule (Cat# AAA2097371)
Full Name
BSA Conjugated Neutrophil Activating Protein 3 (NAP3)
Gene Names
Cxcl1; Gro1; CINC-1
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Activity Data
(Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant human MMP9 (1000ng, 500ng, 100ng, 10ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant human MMP9 (10-70kd) was shown in figure 1.)
Matrix Metalloproteinase 9 (MMP9), Active Protein (Cat# AAA2105102)
Full Name
Active Matrix Metalloproteinase 9 (MMP9)
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $525/0.05 mg | $810/0.1 mg | $1,005/0.2 mg | $1,965/0.5 mg | $2,910/1 mg | $7,115/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD90/Thy1 using anti- CD90/Thy1 antibody (MBS1750721).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: rat thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD. )
CD90/Thy1, Polyclonal Antibody (Cat# AAA1750721)
Full Name
Anti-CD90/Thy1 Picoband Antibody
Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
C ReProtein (CRP), Active Protein (Cat# AAA2105119)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
Purity: >98% as determined by SDS-PAGE.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105112)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Activity Data
(Cluster Of Differentiation 276 (CD276) also known as B7-H3 in human, is a member of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production. Besides, Sialic acid-binding Ig-like lectin 12 (SIGLEC12) has been identified as an interactor of CD276, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD276 and recombinant human SIGLEC12. Briefly, CD276 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC12-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD276 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CD276 and SIGLEC12 was shown in Figure 1, and this effect was in a dose dependent manner.)
Cluster Of Differentiation 276 (CD276), Active Protein (Cat# AAA2097281)
Full Name
Active Cluster Of Differentiation 276 (CD276)
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Sequence
Dipeptidyl Peptidase IV (DPP4), Active Protein (Cat# AAA2105126)
Full Name
Active Dipeptidyl Peptidase IV (DPP4)
Gene Names
DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$205/0.01 mg | $405/0.05 mg | $620/0.1 mg | $765/0.2 mg | $1,490/0.5 mg | $2,215/1 mg | $5,410/5 mg
Activity Data
(Figure. The binding activity of NOS2 with UCHL5.Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Nitric Oxide Synthase 2, Inducible (NOS2), Active Protein (Cat# AAA2105120)
Full Name
Active Nitric Oxide Synthase 2, Inducible (NOS2)
Gene Names
Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Activity Data
(Figure. The binding activity of MCSP with GAL8.Melanoma-associated chondroitin sulfate proteoglycan (MCSP) is a chondroitin sulfate proteoglycan in humans. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. Besides, Galectin-8 (GAL8) has been identified as an interactor of MCSP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MCSP and recombinant human GAL8. Briefly, MCSP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAL8-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MCSP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MCSP and GAL8 was shown in Figure 1, and this effect was in a dose dependent manner.)
Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP), Active Protein (Cat# AAA2105129)
Full Name
Active Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP)
Gene Names
CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Activity Data
(TNFa (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1beta and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL) for 8h, after which the concentration of IL-1beta and IL-8 in the cell supernatant were detected by ELISA. IL-1beta and IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1beta, the data was shown in Figure 1 and Figure 2 separately.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA2097335)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Activity Data
(IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 17 Receptor B (IL17RB), Active Protein (Cat# AAA2097350)
Full Name
Active Interleukin 17 Receptor B (IL17RB)
Gene Names
IL17RB; CRL4; EVI27; IL17BR; IL17RH1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 80%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Activity Data
(High Mobility Group Protein 1 (HMG1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. Besides, Stromal Cell Derived Factor 1 (SDF1) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human SDF1. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SDF1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of HMG1 and SDF1 was shown in Figure 1, and this effect was in a dose dependent manner.)
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA2097253)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Sequence Information
Proline Rich Protein 4, Lacrimal (PRR4), Small Molecule (Cat# AAA2097398)
Full Name
OVA Conjugated Proline Rich Protein 4, Lacrimal (PRR4)
Gene Names
PRR4; LPRP; PROL4
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $805/0.5 mg | $1,180/1 mg | $2,885/5 mg | $12,970/5x5 mg
Activity Data
(IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 6 Receptor (IL6R), Active Protein (Cat# AAA2097235)
Full Name
Active Interleukin 6 Receptor (IL6R)
Gene Names
Il6ra; Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
HPLC of the Original Peptide
Insulin Like Growth Factor 1 (IGF1), Small Molecule (Cat# AAA2097362)
Full Name
OVA Conjugated Insulin Like Growth Factor 1 (IGF1)
Gene Names
IGF1; MGF; IGFI; IGF-I
Applications
Immunogen, Coating Antigen, Blocking Peptide, ELISA.
Purity
>90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Activity Data
(Figure. The binding activity of IL21 with IL2Rg.Interleukin-21 (IL21) is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. This cytokine induces cell division/proliferation in its target cells. IL21 may be a critical factor in the control of persistent viral infections. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL21, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL21 and recombinant human IL2Rg. Briefly, IL21 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL21 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL21 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 21 (IL21), Active Protein (Cat# AAA2105147)
Full Name
Active Interleukin 21 (IL21)
Gene Names
IL21; Za11; IL-21; CVID11
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$200/0.01 mg | $405/0.05 mg | $620/0.1 mg | $765/0.2 mg | $1,480/0.5 mg | $2,205/1 mg | $5,385/5 mg
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097249)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; Bpb; AI850290
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097289)
Full Name
Active Arginase (ARG)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$205/0.01 mg | $420/0.05 mg | $640/0.1 mg | $790/0.2 mg | $1,535/0.5 mg | $2,290/1 mg | $5,590/5 mg
Activity Data
(5'-Nucleotidase, Ecto (NT5E), also known as ecto-5'-nucleotidase or CD73, is an enzyme catalyzing thehydrolysis of nucleoside-5'-monophosphates to nucleosides and inorganic phosphate. The enzyme is a dimer composed of 2 identical 70kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. NT5E is a marker of lymphocyte differentiation that has functions independent of its catalytic activity, such as T-cell activation and cell-cell adhesion. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from NT5E by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The enzyme is widely distributed in human and animal tissues. Besides, AF4/FMR2 Family, Member 1 (AFF1) has been identified as an interactor of NT5E thus a binding ELISA assay was conducted to detect the interaction of recombinant human NT5E and recombinant human AFF1. Briefly, NT5E were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to AFF1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NT5E pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of NT5E and AFF1 was shown in Figure 1, and this effect was in a dose dependent manner.)
5'-Nucleotidase, Ecto (NT5E), Active Protein (Cat# AAA2097292)
Full Name
Active 5'-Nucleotidase, Ecto (NT5E)
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (MBS1750373).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD. )
NFAT2, Polyclonal Antibody (Cat# AAA1750373)
Full Name
Anti-NFAT2 Picoband Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA2097353)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$145/0.01 mg | $250/0.05 mg | $365/0.1 mg | $450/0.2 mg | $850/0.5 mg | $1,250/1 mg | $3,055/5 mg | $13,745/5x5 mg
Western Blot (WB)
(Anti-CEACAM Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CEACAM, Polyclonal Antibody (Cat# AAA9204586)
Full Name
CEACAM Antibody (N-term)
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
