CD80 recombinant antibody

Anti-CD80 [IDEC-114 (Galiximab)]

Cat. Num. AAA488348

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
• Purity: Protein A Affinity Purified
• Synonyms: CD80; Monoclonal Recombinant Antibody; Anti-CD80 [IDEC-114 (Galiximab)]; B7-1; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80 recombinant antibody

• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, Lambda
• Clone Number: IDEC-114
• Specificity: Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.
• Purity/Purification: Protein A Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 288

• Applicable Applications for CD80 recombinant antibody: Block (BL), Modulate, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Galiximab acts to decrease in cell proliferation, inhibition of the constitutively active NF-kappaB pathway, increase in apoptosis and ADCC against various B-cell lymphoma cell lines. Galiximb affects CD28 and CD152 interactions with CD80. This antibody can also be used for FC, WB, ELISA, IP and IHC.
• Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Labeled Substance: None
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

( Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galiximab) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Related Product Information for CD80 recombinant antibody

Recombinant monoclonal antibody to CD80. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody IDEC-114 (Galiximab).
Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)

Product Categories/Family for CD80 recombinant antibody

Non-Hodgekin's Lymphoma; NHL; Cancer; Immunology; Adaptive Immunity; Biosimilar

References

Vinjamaram et al. The use of galiximab in non-Hodgkin lymphoma. Clin Lymphoma Myeloma. 2008 Oct; 8(5):277-82 PMID:18854281

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.4
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 29,413 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.