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10000 results for "Immunity" - showing 650-700
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809870)
Full Name
PI 3 Kinase Class 3 antibody: ATTO 488
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (MBS1750870).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TNFSF18 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD. )
TNFSF18/Gitrl, Polyclonal Antibody (Cat# AAA1750870)
Full Name
Anti-TNFSF18/Gitrl Picoband Antibody
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (MBS1750347).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD. )
Flt3/CD135, Polyclonal Antibody (Cat# AAA1750347)
Full Name
Anti-Flt3/CD135 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(Figure. The binding activity of IL22 with IL10Rb.Interleukin 22 (IL22) is a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL-22 is produced by activated NK and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL22, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL22 and recombinant human IL10Rb. Briefly, IL22 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL22 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL22 and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 22 (IL22), Active Protein (Cat# AAA2105162)
Full Name
Active Interleukin 22 (IL22)
Gene Names
IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Sequence Information
Extracellular Matrix Protein 1 (ECM1), Recombinant Protein (Cat# AAA2097523)
Full Name
Recombinant Extracellular Matrix Protein 1 (ECM1)
Gene Names
ECM1; URBWD
Reactivity
Human
Applications
WB
Purity
> 95%
Pricing
$160/0.01 mg | $290/0.05 mg | $440/0.1 mg | $540/0.2 mg | $1,040/0.5 mg | $1,530/1 mg | $3,745/5 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GIMAP5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
GIMAP5, Polyclonal Antibody (Cat# AAA9130565)
Full Name
GIMAP5 Polyclonal Antibody
Gene Names
GIMAP5; IAN4; IAN5; IROD; IAN-5; IMAP3; HIMAP3; IAN4L1
Reactivity
Human
Applications
WB, IHC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Activity Data
(Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.)
Interleukin 16 (IL16), Active Protein (Cat# AAA2097241)
Full Name
Active Interleukin 16 (IL16)
Gene Names
IL16; LCF; NIL16; PRIL16; prIL-16
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000026)
Full Name
IRGM Antibody: HRP
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Activity Data
(Figure. The binding activity of FGF6 with FGFR1.Fibroblast growth factor 6 (FGF6) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It may also play a importent role in muscle regeneration or differentiation. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF6 and recombinant human FGFR1. Briefly, FGF6 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF6 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibroblast Growth Factor 6 (FGF6), Active Protein (Cat# AAA2105054)
Full Name
Active Fibroblast Growth Factor 6 (FGF6)
Gene Names
FGF6; HST2; HBGF-6
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$155/0.01 mg | $285/0.05 mg | $430/0.1 mg | $525/0.2 mg | $1,010/0.5 mg | $1,490/1 mg | $3,645/5 mg | $16,390/5x5 mg
Activity Data
(Figure 1. The binding activity of ANGPT2 withTie2.Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin cytokines are involved with controlling microvascular permeability, vasodilation, and vasoconstriction by signaling smooth muscle cells surrounding vessels. There are now four identified angiopoietins: ANGPT1, ANGPT2, ANGPT3, ANGPT4. Angiopoietin 2 (ANGPT2) promotes cell death and disrupts vascularization. Yet, when it is in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. Besides, TEK Tyrosine Kinase (Tie2) has been identified as an interactor of ANGPT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANGPT2 and recombinant humanTie2. Briefly, ANGPT2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Tie2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ANGPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ANGPT2 and Tie2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Angiopoietin 2 (ANGPT2), Active Protein (Cat# AAA2105045)
Full Name
Active Angiopoietin 2 (ANGPT2)
Gene Names
ANGPT2; ANG2; AGPT2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $470/0.05 mg | $725/0.1 mg | $900/0.2 mg | $1,750/0.5 mg | $2,610/1 mg | $6,380/5 mg
Western Blot (WB)
CCR7, Polyclonal Antibody (Cat# AAA540572)
Full Name
CCR7 Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, DB, EIA, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
(Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 μg/ml.)
TNFAIP1, Polyclonal Antibody (Cat# AAA154370)
Full Name
TNFAIP1 Antibody
Gene Names
TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097341)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000025)
Full Name
IRGM Antibody: FITC
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD14 using anti-CD14 antibody (MBS1750333).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse spleen tissue lysates,Lane 2: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD14 at approximately 50-55KD. The expected band size for CD14 is at 40KD. )
CD14, Polyclonal Antibody (Cat# AAA1750333)
Full Name
Anti-CD14 Picoband antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg
Western Blot (WB)
(Western blot analysis GIMAP7 using HuvEc whole cell lysates)
GIMAP7, Polyclonal Antibody (Cat# AAA9609864)
Full Name
GIMAP7 Antibody
Gene Names
GIMAP7; IAN7; hIAN7
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809869)
Full Name
PI 3 Kinase Class 3 antibody: ATTO 390
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Recombinant Antibody (Cat# AAA488480)
Full Name
Anti-Tim-4 [5G3]
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Sequence Information
Kallikrein 10 (KLK10), Small Molecule (Cat# AAA2097397)
Full Name
OVA Conjugated Kallikrein 10 (KLK10)
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $340/0.1 mg | $420/0.2 mg | $795/0.5 mg | $1,165/1 mg | $2,840/5 mg | $12,775/5x5 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD62L antibody DREG-56 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of DREG-56 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488437)
Full Name
Anti-CD62L [DREG-56]
Gene Names
SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
Reactivity
Human, Chimpanzee, Bovine
Applications
IP, FC/FACS, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000274)
Full Name
Cyclooxygenase 1 Antibody: Alkaline Phosphatase
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Activity Data
(Figure. Cell proliferation of A549 cells after stimulated with GREM1.Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant human GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GREM1 for 96h. The result was shown in Figure 2. It was obvious that GREM1 significantly increased cell viability of A549 cells.(A) A549 cells cultured in DMEM, stimulated with 1ng/mL GREM1 for 96h; (B) Unstimulated A549 cells cultured in DMEM for 96h.)
Gremlin 1 (GREM1), Active Protein (Cat# AAA2105165)
Full Name
Active Gremlin 1 (GREM1)
Gene Names
GREM1; DRM; HMPS; MPSH; PIG2; CRAC1; CRCS4; DAND2; HMPS1; IHG-2; DUP15q; C15DUPq; GREMLIN; CKTSF1B1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000024)
Full Name
IRGM Antibody: Biotin
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809879)
Full Name
PI 3 Kinase Class 3 antibody: Biotin
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Activity Data
(Figure. The binding activity of IP10 with IGFBP7.Interferon gamma-induced protein 10 (IP10) also known as C-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10 is an 8.7kDa protein that in humans is encoded by the CXCL10 gene. C-X-C motif chemokine 10 is a small cytokine belonging to the CXC chemokine family. IP10 secreted by several cell types in response to IFN-gamma, has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. Besides, Insulin Like Growth Factor Binding Protein 7 (IGFBP7) has been identified as an interactor of IP10, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IP10 and recombinant human IGFBP7. Briefly, VDR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IGFBP7-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IP10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IP10 and IGFBP7 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Gamma Induced Protein 10kDa (IP10), Active Protein (Cat# AAA2105090)
Full Name
Active Interferon Gamma Induced Protein 10kDa (IP10)
Gene Names
CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $480/0.05 mg | $740/0.1 mg | $915/0.2 mg | $1,790/0.5 mg | $2,645/1 mg | $6,465/5 mg
Sequence Information
Mucin 5 Subtype B (MUC5B), Small Molecule (Cat# AAA2097358)
Full Name
OVA Conjugated Mucin 5 Subtype B (MUC5B)
Gene Names
MUC5B; MG1; MUC5; MUC9; MUC-5B
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$135/0.01 mg | $225/0.05 mg | $315/0.1 mg | $395/0.2 mg | $745/0.5 mg | $1,090/1 mg | $2,660/5 mg | $11,960/5x5 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488448)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(IL17B (Interleukin-17B) is is a T cell-derived cytokine that shares sequence similarity with IL17, which has been proven to mediate potent inflammatory immune response. Besides, increasing evidence suggests that IL17 may play a critical role in various kinds of liver diseases, including hepatocellualar carcinoma. Thus a stimulation assay has been conducted to detect the bioactivity of IL17B. HepG2 cells were seeded into 24-well plate at a density of 1x10^5 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (250ng/mL, 500ng/mL) of IL17B for 20h and IL-8 levels in the cell supernatant were determined by ELISA.)
Interleukin 17B (IL17B), Active Protein (Cat# AAA2097329)
Full Name
Active Interleukin 17B (IL17B)
Gene Names
IL17B; NIRF; IL-20; IL-17B; ZCYTO7
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$170/0.01 mg | $320/0.05 mg | $500/0.1 mg | $615/0.2 mg | $1,180/0.5 mg | $1,755/1 mg | $4,310/5 mg
CD80, Monoclonal Recombinant Antibody (Cat# AAA488347)
Full Name
Anti-CD80 [IDEC-114 (Galiximab)]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, FC/FACS, WB, EIA, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809971)
Full Name
RIP3 Antibody: HRP
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Sequence Information
Toll Like Receptor 4 (TLR4), Small Molecule (Cat# AAA2097394)
Full Name
OVA Conjugated Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$145/0.01 mg | $245/0.05 mg | $360/0.1 mg | $440/0.2 mg | $830/0.5 mg | $1,220/1 mg | $2,980/5 mg | $13,395/5x5 mg
Activity Data
(IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL 2. Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 2 Receptor Alpha (IL2Ra), Active Protein (Cat# AAA2097348)
Full Name
Active Interleukin 2 Receptor Alpha (IL2Ra)
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Activity Data
(FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 1, Acidic (FGF1), Active Protein (Cat# AAA2097239)
Full Name
Active Fibroblast Growth Factor 1, Acidic (FGF1)
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $800/0.5 mg | $1,175/1 mg | $2,865/5 mg | $12,890/5x5 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD81 antibody 1D6 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
CD81, Monoclonal Recombinant Antibody (Cat# AAA488456)
Full Name
Anti-CD81 [1D6]
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Goat, Sheep
Applications
EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 (MBS488449) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488449, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
alpha-4 Integrin, Monoclonal Recombinant Antibody (Cat# AAA488449)
Full Name
Anti-alpha-4 Integrin [21.6]
Gene Names
ITGA4; IA4; CD49D
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809974)
Full Name
RIP3 Antibody: RPE
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Testing Data
(Surface plasmon resonance (SPR) sensorgram anti-TIM-1 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein (Pr00227-1.9) to immbolised the rabbit IgG chimeric version of 3B3. Pr00227-1.9 was injected and allowed to associate with between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. Pr00227-1.9 showed rapid binding to and following ligand removal remained strongly bound. This suggests that has a very high affinity for Pr00227-1.9.)
Tim-1, Monoclonal Recombinant Antibody (Cat# AAA488475)
Full Name
Anti-Tim-1 [3B3]
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Sequence Information
Gelsolin (GS), Small Molecule (Cat# AAA2097364)
Full Name
OVA Conjugated Gelsolin (GS)
Gene Names
GSN; ADF; AGEL
Reactivity
Human
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $230/0.05 mg | $335/0.1 mg | $415/0.2 mg | $780/0.5 mg | $1,145/1 mg | $2,800/5 mg | $12,580/5x5 mg
Sequence Information
Interleukin 1 Beta (IL1b), Small Molecule (Cat# AAA2097361)
Full Name
BSA Conjugated Interleukin 1 Beta (IL1b)
Gene Names
Il1b; IL-1F2
Reactivity
Rat
Applications
WB
Purity
> 90%
Pricing
$140/0.01 mg | $240/0.05 mg | $345/0.1 mg | $425/0.2 mg | $805/0.5 mg | $1,180/1 mg | $2,885/5 mg | $12,970/5x5 mg
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Brain Tissue; Lane3: Mouse Liver Tissue; Lane4: Human A549 Cells.)
Heat Shock 70kDa Protein 1A (HSPA1A), Polyclonal Antibody (Cat# AAA2034675)
Full Name
Anti-Heat Shock 70kDa Protein 1A (HSPA1A) Polyclonal Antibody
Gene Names
HSPA1B; HSP70-2; HSP70.2; HSP70-1B
Reactivity
Human
Applications
WB
Purity
Antigen-specific affinity chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant ERK1, Human.)
Extracellular Signal Regulated Kinase 1 (ERK1), Polyclonal Antibody (Cat# AAA2034685)
Full Name
Anti-Extracellular Signal Regulated Kinase 1 (ERK1) polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
WB
Purity
Antigen-specific affinity chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $350/0.5 mL | $430/1 mL | $1,235/5x1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809960)
Full Name
RIP3 Antibody: ATTO 488
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of IL-12A in HeLa cell lysate with IL-12A antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
IL-12A, Polyclonal Antibody (Cat# AAA154392)
Full Name
IL-12A Antibody
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Reactivity
Human
Applications
EIA, WB
Purity
Affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809959)
Full Name
RIP3 Antibody: ATTO 390
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunohistochemistry (IHC)
((Figure: FITC staining on IHC-P; Samples: Rat Kidney Tissue.))
IgG, Polyclonal Secondary Antibody (Cat# AAA2086043)
Full Name
FITC-Linked Rabbit Anti-Rat IgG Polyclonal Antibody
Reactivity
Rat
Applications
WB, IHC, ICC, IF
Purity
Antigen-specific affinity chromatography
Pricing
$80/0.1 mL | $85/0.2 mL | $115/0.5 mL | $120/1 mL | $440/5x1 mL
Flow Cytometry (FC/FACS)
(Fig-1: Immunohistochemical analysis of TLR5 in human skin tissue using TLR5 antibody (Clone: ABM22G1) at 10 ug/ml.)
TLR5, Monoclonal Antibody (Cat# AAA668960)
Full Name
NALE Monoclonal Antibody to TLR5 (Clone: ABM22G1) (No Azide Low Endotoxin)
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000278)
Full Name
Cyclooxygenase 1 Antibody: HRP
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD44 antibody Hermes-1 Human activated T lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-1 (MBS488445, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD44, Monoclonal Recombinant Antibody (Cat# AAA488445)
Full Name
Anti-CD44 [Hermes-1]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
Applications
BL, EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (MBS488479) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488479, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Recombinant Antibody (Cat# AAA488479)
Full Name
Anti-Tim-4 [5G3]
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 (MBS488441) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488441, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488441)
Full Name
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
IF, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
