Activity Data
(Figure. The binding activity of Slit2 with GREM1.Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Slit Homolog 2 (Slit2) Active Protein | Slit2 active protein
Active Slit Homolog 2 (Slit2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Activity Data
(Figure. The binding activity of Slit2 with GREM1.Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Figure. The binding activity of Slit2 with GREM1.Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.)
NCBI and Uniprot Product Information
Accurate MW: 20kDa
NCBI Description
acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [RGD, Feb 2006]
Research Articles on Slit2
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Product Notes
The Slit2 slit2 (Catalog #AAA2105109) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Asn209~Gly374. AAA Biotech's Slit Homolog 2 (Slit2) can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the Slit2 slit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Slit Homolog 2 (Slit2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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