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Gene Name
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10000 results for "Immunity" - showing 400-450
Typical Testing Data/Standard Curve (for reference only)
Interleukin 25 (IL25), ELISA Kit (Cat# AAA2023946)
Full Name
Human Interleukin 25 (IL25) ELISA Kit
Gene Names
IL25; IL17E
Reactivity
Human
Pricing
$510/48-Strip-Wells | $685/96-Strip-Wells | $2,745/5x96-Strip-Wells | $5,005/10x96-Strip-Wells | $340/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Monocyte Chemotactic Protein 1 (MCP1), ELISA Kit (Cat# AAA2025214)
Full Name
Mouse Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
Reactivity
Mouse
Pricing
$500/48-Strip-Wells | $670/96-Strip-Wells | $2,685/5x96-Strip-Wells | $4,900/10x96-Strip-Wells | $335/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Albumin (ALB), ELISA Kit (Cat# AAA2022813)
Full Name
Rat Albumin (ALB) ELISA Kit
Gene Names
ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
Reactivity
Rat
Pricing
$350/48-Strip-Wells | $460/96-Strip-Wells | $1,850/5x96-Strip-Wells | $3,375/10x96-Strip-Wells | $255/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 4, ELISA Kit (Cat# AAA702795)
Full Name
Pig interleukin 4, IL-4 ELISA Kit
Reactivity
Pig
Pricing
$405/48-Strip-Wells | $570/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,470/5x96-Strip-Wells | $4,700/10x96-Strip-Wells
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue.)
Catenin Beta 1 (CTNNb1), Polyclonal Antibody (Cat# AAA2034673)
Full Name
Anti-Catenin Beta 1 (CTNNb1) polyclonal Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human
Applications
WB
Purity
Antigen-specific affinity chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $350/0.5 mL | $430/1 mL | $1,235/5x1 mL
Typical Testing Data/Standard Curve (for reference only)
Keratin 4 (KRT4), ELISA Kit (Cat# AAA2020634)
Full Name
Human Keratin 4 (KRT4) ELISA Kit
Gene Names
KRT4; K4; CK4; CK-4; CYK4; WSN1
Reactivity
Human
Pricing
$490/48-Strip-Wells | $655/96-Strip-Wells | $2,625/5x96-Strip-Wells | $4,815/10x96-Strip-Wells | $330/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Epiregulin (EREG), ELISA Kit (Cat# AAA2020182)
Full Name
Human Epiregulin (EREG) ELISA Kit
Gene Names
EREG; ER; Ep; EPR
Reactivity
Human
Pricing
$510/48-Strip-Wells | $685/96-Strip-Wells | $2,745/5x96-Strip-Wells | $5,005/10x96-Strip-Wells | $340/24-Strip-Wells
Activity Data
(IL7 (Interleukin 7) is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. The interaction between IL17 and the IL7 receptor triggers a cascade of signals important for T-cell development within the thymus and survival within the periphery. It is reported that IL-7 acts on both resting and activated T cells, including Jurkat cells. Thus, a proliferation assay of recombinant human IL7 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL7 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL7 significantly promoted cell proliferation of Jurkat cells.)
Interleukin 7 (IL7), Active Protein (Cat# AAA2097240)
Full Name
Active Interleukin 7 (IL7)
Gene Names
IL7; IL-7
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $510/0.05 mg | $795/0.1 mg | $985/0.2 mg | $1,920/0.5 mg | $2,840/1 mg | $6,945/5 mg
Typical Testing Data/Standard Curve (for reference only)
Fibulin 1 (FBLN1), ELISA Kit (Cat# AAA2023994)
Full Name
Human Fibulin 1 (FBLN1) ELISA Kit
Gene Names
FBLN1; FBLN; FIBL1
Reactivity
Human
Pricing
$535/48-Strip-Wells | $710/96-Strip-Wells | $2,870/5x96-Strip-Wells | $5,220/10x96-Strip-Wells | $345/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Mucin 1 (MUC1), ELISA Kit (Cat# AAA2021290)
Full Name
Human Mucin 1 (MUC1) ELISA Kit
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Pricing
$445/48-Strip-Wells | $590/96-Strip-Wells | $2,395/5x96-Strip-Wells | $4,375/10x96-Strip-Wells | $305/24-Strip-Wells
Immunohistochemistry (IHC)
(Formalin-paraffin human Colon Carcinoma stained with IgA Secretory Component Mab (SPM217).)
IgA Secretory Component / ECM1, Monoclonal Antibody (Cat# AAA438012)
Full Name
IgA Secretory Component / ECM1 Mouse Monoclonal Antibody
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA2097256)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
Ifna1; Ifa1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fc gamma receptor III antibody 3G8 (Ab00754) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of 3G8 (Ab00754-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488350)
Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Typical Testing Data/Standard Curve (for reference only)
Haptoglobin (Hpt), ELISA Kit (Cat# AAA2024822)
Full Name
Bovine Haptoglobin (Hpt) ELISA Kit
Gene Names
HP; BP; HPA1S; HP2ALPHA2
Reactivity
Bovine
Pricing
$565/48-Strip-Wells | $760/96-Strip-Wells | $3,060/5x96-Strip-Wells | $5,585/10x96-Strip-Wells | $365/24-Strip-Wells
Immunohistochemistry (IHC)
(MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)
CSF1R, Monoclonal Antibody (Cat# AAA9200563)
Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA700332)
Full Name
Rat Interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; TCGF
Reactivity
Rat
Pricing
$345/48-Strip-Wells | $475/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,050/5x96-Strip-Wells | $3,915/10x96-Strip-Wells
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000281)
Full Name
Cyclooxygenase 1 Antibody: RPE
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).)
IgA Secretory Component / ECM1, Monoclonal Antibody (Cat# AAA438891)
Full Name
IgA Secretory Component / ECM1 Mouse Monoclonal Antibody
Gene Names
ECM1; URBWD
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Typical Testing Data/Standard Curve (for reference only)
Interleukin 13 (IL13), ELISA Kit (Cat# AAA2022679)
Full Name
Rat Interleukin 13 (IL13) ELISA Kit
Gene Names
IL13; P600; IL-13
Reactivity
Rat
Pricing
$525/48-Strip-Wells | $695/96-Strip-Wells | $2,810/5x96-Strip-Wells | $5,125/10x96-Strip-Wells | $345/24-Strip-Wells
Activity Data
(IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.)
Interleukin 11 (IL11), Active Protein (Cat# AAA2097260)
Full Name
Active Interleukin 11 (IL11)
Gene Names
IL11; AGIF; IL-11
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105113)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
Mif; GIF; DER6; Glif
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Sequence
Galectin 3 (GAL3), Active Protein (Cat# AAA2105089)
Full Name
Active Galectin 3 (GAL3)
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Typical Testing Data/Standard Curve (for reference only)
fibronectin 1, ELISA Kit (Cat# AAA703777)
Full Name
Rat Fibronectin, FN ELISA Kit
Reactivity
Rat
Pricing
$530/48-Strip-Wells | $755/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,875/5x96-Strip-Wells | $5,480/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 27, ELISA Kit (Cat# AAA703447)
Full Name
Mouse Interleukin 27, IL-27 ELISA Kit
Gene Names
Il27; p28; Il30; IL-27; IL-27p28
Reactivity
Mouse
Pricing
$405/48-Strip-Wells | $570/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,475/5x96-Strip-Wells | $4,710/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 8, ELISA Kit (Cat# AAA902537)
Full Name
rabbit Interleukin 8, IL-8 ELISA Kit
Gene Names
CXCL8; IL8
Reactivity
Rabbit
Pricing
$615/48-Strip-Wells | $890/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $3,185/5x96-Strip-Wells | $6,110/10x96-Strip-Wells
Activity Data
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.)
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA2097263)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$205/0.01 mg | $415/0.05 mg | $635/0.1 mg | $790/0.2 mg | $1,530/0.5 mg | $2,280/1 mg | $5,565/5 mg
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA701474)
Full Name
Pig interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; IL-2; TCGF; POIL2
Reactivity
Pig
Pricing
$425/48-Strip-Wells | $595/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,555/5x96-Strip-Wells | $4,865/10x96-Strip-Wells
Western Blot (WB)
(Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).)
Leptin Receptor (LEPR), Polyclonal Antibody (Cat# AAA9204850)
Full Name
Leptin Receptor (LEPR) Antibody (N-term)
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
interleukin 8, ELISA Kit (Cat# AAA700388)
Full Name
Pig interleukin 8, IL-8 ELISA Kit
Gene Names
IL8; CXCL8; AMCF-I
Reactivity
Pig
Pricing
$405/48-Strip-Wells | $570/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,470/5x96-Strip-Wells | $4,700/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 4, ELISA Kit (Cat# AAA700367)
Full Name
Rat Interleukin 4, IL-4 ELISA Kit
Gene Names
IL4; IL2; BSF-1
Reactivity
Rat
Pricing
$345/48-Strip-Wells | $475/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,050/5x96-Strip-Wells | $3,915/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA704522)
Full Name
Mouse Interleukin 2, IL-2 ELISA Kit
Gene Names
Il2; Il-2
Reactivity
Mouse
Pricing
$345/48-Strip-Wells | $475/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,050/5x96-Strip-Wells | $3,915/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Syndecan 1 (SDC1), ELISA Kit (Cat# AAA2019461)
Full Name
Mouse Syndecan 1 (SDC1) ELISA Kit
Gene Names
SDC1; SDC; CD138; SYND1; syndecan
Reactivity
Mouse
Pricing
$525/48-Strip-Wells | $695/96-Strip-Wells | $2,810/5x96-Strip-Wells | $5,125/10x96-Strip-Wells | $345/24-Strip-Wells
Activity Data
(Figure. The binding activity of IGF1 with IGFBP3.Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. Besides, Insulin Like Growth Factor Binding Protein 3 (IGFBP3) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGF1 and recombinant mouse IGFBP3. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IGFBP3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IGF1 and IGFBP3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin Like Growth Factor 1 (IGF1), Active Protein (Cat# AAA2105063)
Full Name
Active Insulin Like Growth Factor 1 (IGF1)
Gene Names
Igf1; Igf-1; Igf-I; C730016P09Rik
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$105/0.01 mg | $160/0.05 mg | $215/0.1 mg | $250/0.2 mg | $450/0.5 mg | $645/1 mg | $1,555/5 mg | $6,995/5x5 mg
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, rat RAGE shares similarities with human RAGE in amino acids sequence with the identity of 80.0%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097246)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; S100P
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Activity Data
(PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Parathyroid Hormone Related Protein (PTHrP), Active Protein (Cat# AAA2097284)
Full Name
Active Parathyroid Hormone Related Protein (PTHrP)
Gene Names
Pthlh; PLP; Pthrp; PTH-like
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (MBS1750396).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD. )
IL17, Polyclonal Antibody (Cat# AAA1750396)
Full Name
Anti-IL17 Picoband Antibody
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
Osteopontin (OPN), ELISA Kit (Cat# AAA2024631)
Full Name
Human Osteopontin (OPN) ELISA Kit
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Pricing
$385/48-Strip-Wells | $515/96-Strip-Wells | $2,065/5x96-Strip-Wells | $3,790/10x96-Strip-Wells | $280/24-Strip-Wells
Activity Data
(Figure. The chemotactic effect of NAP3 on THP-1 cells.Neutrophil-activating protein 3 (NAP3) also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 is expressed by macrophages, neutrophils and epithelial cells,and has neutrophil chemoattractant activity. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (31.25ng/mL, 62.5ng/mL,125ng/mL and 250ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of NAP3 occurs at 31.25-250ng/mL.(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 62.5ng/mL NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h; (B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.)
Neutrophil Activating Protein 3 (NAP3), Active Protein (Cat# AAA2105058)
Full Name
Active Neutrophil Activating Protein 3 (NAP3)
Gene Names
CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$145/0.01 mg | $255/0.05 mg | $375/0.1 mg | $460/0.2 mg | $870/0.5 mg | $1,280/1 mg | $3,125/5 mg | $14,055/5x5 mg
Sequence
Asporin (ASPN), Active Protein (Cat# AAA2105172)
Full Name
Active Asporin (ASPN)
Gene Names
Aspn; Plap1; Slrr1c; AA986886; 4631401G09Rik
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$210/0.01 mg | $435/0.05 mg | $665/0.1 mg | $825/0.2 mg | $1,600/0.5 mg | $2,385/1 mg | $5,820/5 mg
Activity Data
(Figure. The binding activity of TGM1 with SNCa.Transglutaminase 1(TGM1) is an enzyme found in cells that make up the outermost layer of the skin (the epidermis). It can catalyze the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. TGM1 is involved in the formation of the cornified cell envelope, which is a structure that surrounds skin cells and helps form a protective barrier between the body and its environment. Besides, Synuclein Alpha (SNCa) has been identified as an interactor of TGM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TGM1 and recombinant mouse SNCa. Briefly, TGM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SNCa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TGM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TGM1 and SNCa was shown in Figure 1, and this effect was in a dose dependent manner.)
Transglutaminase 1, Keratinocyte (TGM1), Active Protein (Cat# AAA2105150)
Full Name
Active Transglutaminase 1, Keratinocyte (TGM1)
Gene Names
Tgm1; Tgase1; 2310004J08Rik
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $505/0.05 mg | $785/0.1 mg | $970/0.2 mg | $1,895/0.5 mg | $2,805/1 mg | $6,860/5 mg
Activity Data
(Figure. Cell proliferation of TF-1 cells after stimulated with LIF.Leukemia Inhibitory Factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. To test the effect of LIF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human LIF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with LIF for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant LIF for 72h. The result was shown in Figure 2. It was obvious that LIF significantly increased cell viability of TF-1 cells.(A) TF-1 cells cultured in 1640, stimulated with 0.5ng/mL LIF for 72h; (B) Unstimulated TF-1 cells cultured in 1640 for 72h.)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA2097262)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Western Blot (WB)
(IRF9 Antibody (N-term)(MBS9209363) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).)
IRF9, Polyclonal Antibody (Cat# AAA9209363)
Full Name
IRF9 Antibody (N-term)
Gene Names
IRF9; p48; IRF-9; ISGF3; ISGF3G
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Sequence)
Fibronectin (FN), Active Protein (Cat# AAA2097257)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Applications
Cell culture, Activity Assays
Purity
>98%
Pricing
$175/0.01 mg | $335/0.05 mg | $510/0.1 mg | $625/0.2 mg | $1,205/0.5 mg | $1,790/1 mg | $4,385/5 mg
Sequence
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA2097250)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Applications
Cell culture; Activity Assays; In vivo assays
Purity
>95%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $675/0.2 mg | $1,310/0.5 mg | $1,950/1 mg | $4,785/5 mg
CD284, Monoclonal Antibody (Cat# AAA438978)
Full Name
CD284 (Toll-Like Receptor 4) Mouse Monoclonal Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Monkey, Pig, Dog, Rat, Guinea Pig
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Plasma Cell Marker Monoclonal Antibody (SPM310).)
Plasma Cell Marker, Monoclonal Antibody (Cat# AAA438283)
Full Name
Plasma Cell Marker Mouse Monoclonal Antibody
Reactivity
Human.
Does not react with Rat
Does not react with Rat
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Sequence Information
Interleukin 10 Receptor Beta (IL10Rb), Active Protein (Cat# AAA2097221)
Full Name
Active Interleukin 10 Receptor Beta (IL10Rb)
Gene Names
IL10RB; CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
Reactivity
Homo sapiens (Human)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$180/0.01 mg | $350/0.05 mg | $535/0.1 mg | $655/0.2 mg | $1,265/0.5 mg | $1,880/1 mg | $4,610/5 mg
Activity Data
(Figure. The binding activity of ODC with TK1.Ornithine decarboxylase (ODC) is an enzyme can catalyze the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. The ornithine decarboxylation reaction catalyzed by ornithine decarboxylase is the first and committed step in the synthesis of polyamines, particularly putrescine, spermidine and spermine. Polyamines are important for stabilizing DNA structure, the DNA double strand-break repair pathway and as antioxidants. Therefore, ornithine decarboxylase is an essential enzyme for cell growth, producing the polyamines necessary to stabilize newly synthesized DNA. Lack of ODC causes cell apoptosis in embryonic mice, induced by DNA damage. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ODC and recombinant human TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105137)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
ODC1; ODC
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Sequence Information
Epidermal Growth Factor (EGF), Recombinant Protein (Cat# AAA2097548)
Full Name
Recombinant Epidermal Growth Factor (EGF)
Gene Names
Egf; AI790464
Reactivity
Mouse
Applications
WB
Purity
> 95%
Pricing
$145/0.01 mg | $250/0.05 mg | $365/0.1 mg | $450/0.2 mg | $850/0.5 mg | $1,250/1 mg | $3,055/5 mg | $13,745/5x5 mg
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097233)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Gene Names
IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
