Rat NCAM1/CD56 ELISA Kit | NCAM1 elisa kit

Rat NCAM1/CD56 PicoKine ELISA Kit

Cat. Num. AAA178749

• Gene Names: Ncam1; Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• Reactivity: Rat
• Synonyms: NCAM1/CD56; Rat NCAM1/CD56 PicoKine ELISA Kit; CD56; NCAM 1; NCAM C; NCAM1; NCAM-1; N-CAM-1; Neural cell adhesion molecule 1; NCAM1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CD56
• Sequence Length: 858

• Assay Range: 625pg/ml-40000pg/ml
• Sensitivity: <20pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: NSO, L20-T712
• Sample Data: Concentration(pg/ml): |0|625|1250|2500|5000|10000|20000|40000; O.D.: |0.004|0.064|0.118|0.215|0.450|0.798|1.578|2.317
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(ng/ml)|1.32|5.61|25.58|1.54|6.32|30.32; Standard Deviation: |0.057|0.309|1.688|0.074|0.430|2.213; CV(%): |4.3|5.5|6.6|4.8|6.8|7.3
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 40000pg/ml, 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml rat CD56 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat CD56 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat NCAM1/CD56 PicoKine ELISA Kit standard curve )

Related Product Information for NCAM1 elisa kit

For quantitative detection of rat CD56 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. And the encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
Principle: Rat CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for CD56 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L20-T712) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CD56 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 13928706
• NCBI GeneID: 24586
• NCBI Accession #: NP_113709.1
• NCBI GenBank Nucleotide #: NM_031521.1
• UniProt Accession #: P13596
• Molecular Weight: 94,658 Da
• NCBI Official Full Name: neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: Ncam1
• NCBI Official Synonym Symbols: Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: Ncam1
• UniProt Synonym Gene Names: Ncam; N-CAM-1; NCAM-1

NCBI Description

cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: axon; cell soma; cell surface; cytoplasm; external side of plasma membrane; growth cone; intercellular junction; membrane; myelin sheath; plasma membrane

Molecular Function: cytoskeletal protein binding; fibroblast growth factor receptor binding; identical protein binding; LRR domain binding; phosphatase binding; protein binding

Biological Process: aging; axon guidance; axon regeneration in the peripheral nervous system; calcium-independent cell-cell adhesion; cell adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; learning and/or memory; multicellular organismal response to stress; neurite development; neuron development; organ regeneration; positive regulation of calcium-mediated signaling; positive regulation of cardiac muscle cell proliferation; regulation of exocyst assembly; regulation of sensory perception of pain; response to cocaine; response to drug; response to inorganic substance; response to lead ion; thalamus development