Rat CD56 ELISA Kit | NCAM1 elisa kit

Rat CD56 ELISA Kit

Cat. Num. AAA8248485

• Gene Names: Ncam1; Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• Reactivity: Rat
• Synonyms: CD56; Rat CD56 ELISA Kit; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; NCAM1 elisa kit

• Reactivity: Rat
• Specificity: The Rat CD56 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CD56 proteins within the range of 625 pg/ml - 40000 pg/ml.
• Sequence Length: 858

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <20 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCAM1 elisa kit

Principle of the Assay: The Rat CD56 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD56 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat CD56 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD56 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD56 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD56 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. And the encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

NCBI and Uniprot Product Information

• NCBI GI #: 13928706
• NCBI GeneID: 24586
• NCBI Accession #: NP_113709.1
• NCBI GenBank Nucleotide #: NM_031521.1
• UniProt Accession #: P13596
• Molecular Weight: 94,658 Da
• NCBI Official Full Name: neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: Ncam1
• NCBI Official Synonym Symbols: Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: Ncam1
• UniProt Synonym Gene Names: Ncam; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_RAT

NCBI Description

cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: axon; cell soma; cell surface; cytoplasm; external side of plasma membrane; growth cone; intercellular junction; membrane; myelin sheath; plasma membrane

Molecular Function: cytoskeletal protein binding; fibroblast growth factor receptor binding; identical protein binding; LRR domain binding; phosphatase binding; protein binding

Biological Process: aging; axon guidance; axon regeneration in the peripheral nervous system; calcium-independent cell-cell adhesion; cell adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; learning and/or memory; multicellular organismal response to stress; neurite development; neuron development; organ regeneration; positive regulation of calcium-mediated signaling; positive regulation of cardiac muscle cell proliferation; regulation of exocyst assembly; regulation of sensory perception of pain; response to cocaine; response to drug; response to inorganic substance; response to lead ion; thalamus development