Typical Testing Data/Standard Curve (for reference only)
(Rat Endostatin PicoKine ELISA Kit standard curve )
Rat Endostatin ELISA Kit | COL18A1 elisa kit
Rat Endostatin PicoKine ELISA Kit
Reactivity
Rat
Synonyms
Endostatin; Rat Endostatin PicoKine ELISA Kit; Alpha 1 type XVIII collagen; COIA1; COL18A1; Collagen alpha 1(XVIII) chain; Collagen alpha1(XVIII) chain; Collagen alpha-1(XVIII) chain; Collagen type XVIII alpha 1; collagen; type XVIII; alpha 1; KNO 1; KNO1; P39060; COL18A1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat Endostatin
Sequence Length
171
Assay Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
25-30
Immunogen
NSO, H1591-K1774
Sample Data
Concentration(pg/ml): |0|156|312|625|1250|2500|5000|10000
O.D.: |0.032|0.175|0.294|0.511|0.851|1.320|1.825|2.213
O.D.: |0.032|0.175|0.294|0.511|0.851|1.320|1.825|2.213
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |339|1447|6124|415|1829|6450
Standard Deviation: |18.65|91.16|440.93|25.32|120.71|503.10
CV(%): |5.5|6.3|7.2|6.1|6.6|7.8
n: |16|16|16|24|24|24
Mean(pg/ml): |339|1447|6124|415|1829|6450
Standard Deviation: |18.65|91.16|440.93|25.32|120.71|503.10
CV(%): |5.5|6.3|7.2|6.1|6.6|7.8
Assay Procedure Step 1
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat Endostatin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat Endostatin standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Rat Endostatin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat Endostatin PicoKine ELISA Kit standard curve )
Related Product Information for COL18A1 elisa kit
For quantitative detection of rat Endostatin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Principle: Rat Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Endostatin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Endostatin amount of sample captured in plate.
Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Principle: Rat Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Endostatin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Endostatin amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
18,933 Da
NCBI Official Full Name
Collagen type XVIII, alpha (I) chain
NCBI Official Synonym Full Names
collagen type XVIII alpha 1 chain
NCBI Official Symbol
Col18a1
NCBI Protein Information
collagen alpha-1(XVIII) chain
UniProt Protein Name
Collagen type XVIII, alpha (I) chain
Protein Family
UniProt Gene Name
Col18a1
NCBI Description
intermediate filament protein; a precursor of the angiogenesis inhibitor endostatin [RGD, Feb 2006]
Research Articles on COL18A1
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Product Notes
The Rat COL18A1 col18a1 (Catalog #AAA178727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178727 ELISA Kit recognizes Rat COL18A1. It is sometimes possible for the material contained within the vial of "Endostatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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