NCAM1 / CD56 recombinant protein

Recombinant Rat NCAM1 / CD56 Protein (His tag)

Cat. Num. AAA2547302

• Gene Names: Ncam1; Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: NCAM1 / CD56; Recombinant Rat NCAM1 / CD56 Protein (His tag); NCAM1; Ncam; NCAM1 / CD56 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50 mM Tris, 0.15M NaCl, pH 8.0
• Sequence Length: 858

• Application Notes: The recombinant rat NCAM1 comprises 713 amino acids and predicts a molecular mass of 79.2 kDa. The apparent molecular mass of the recombinant protein is approximately 114-118 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Leu 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NCAM1 / CD56 recombinant protein

Background: NCAM1, also known as CD56, is a neural adhesion protein (NCAM) which belongs to the immunoglobulin superfamily. NCAM is involved in neural development and in plasticity in the adult brain. UCHL1 is a novel interaction partner of both NCAM isoforms that regulates their ubiquitination and intracellular trafficking. NCAM1 is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. NCAM1 has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Description: A DNA sequence encoding the rat NCAM1 (P13596-1) (Met1-Thr721) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13928706
• NCBI GeneID: 24586
• NCBI Accession #: NP_113709.1
• NCBI GenBank Nucleotide #: NM_031521.1
• UniProt Accession #: P13596
• Molecular Weight: 94,658 Da
• NCBI Official Full Name: neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: Ncam1
• NCBI Official Synonym Symbols: Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• UniProt Gene Name: Ncam1
• UniProt Synonym Gene Names: Ncam; N-CAM-1; NCAM-1

NCBI Description

cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: axon; cell soma; cell surface; cytoplasm; external side of plasma membrane; growth cone; intercellular junction; membrane; myelin sheath; plasma membrane

Molecular Function: cytoskeletal protein binding; fibroblast growth factor receptor binding; identical protein binding; LRR domain binding; phosphatase binding; protein binding

Biological Process: aging; axon guidance; axon regeneration in the peripheral nervous system; calcium-independent cell-cell adhesion; cell adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; learning and/or memory; multicellular organismal response to stress; neurite development; neuron development; organ regeneration; positive regulation of calcium-mediated signaling; positive regulation of cardiac muscle cell proliferation; regulation of exocyst assembly; regulation of sensory perception of pain; response to cocaine; response to drug; response to inorganic substance; response to lead ion; thalamus development