Typical Testing Data/Standard Curve (for reference only)
(Rat WIF1 PicoKine ELISA Kit standard curve )
Rat WIF1 ELISA Kit | WIF1 elisa kit
Rat WIF1 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
WIF1; Rat WIF1 PicoKine ELISA Kit; WIF-1; Wnt inhibitory factor 1; Q9Y5W5; WIF1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat WIF1
Sequence Length
365
Assay Range
23.4pg/ml-1500pg/ml
Sensitivity
<10pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
25-30
Immunogen
NSO, G29-W379
Sample Data
Concentration(pg/ml): |0|23.4|46.9|93.8|187.5|375|750|1500
O.D.: |0.161|0.207|0.211|0.303|0.440|0.729|1.266|2.369
O.D.: |0.161|0.207|0.211|0.303|0.440|0.729|1.266|2.369
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |50.3|212|894|62.1|258|903
Standard Deviation: |2.11|10.60|51.85|3.42|16.51|70.43
CV(%): |4.2|5.0|5.8|5.5|6.4|7.8
n: |16|16|16|24|24|24
Mean(pg/ml): |50.3|212|894|62.1|258|903
Standard Deviation: |2.11|10.60|51.85|3.42|16.51|70.43
CV(%): |4.2|5.0|5.8|5.5|6.4|7.8
Assay Procedure Step 1
Aliquot 0.1ml per well of the 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml, 23.4pg/ml rat WIF1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each rat WIF1 standard solution and each sample is measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Rat WIF1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat WIF1 PicoKine ELISA Kit standard curve )
Related Product Information for WIF1 elisa kit
For quantitative detection of rat WIF1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Principle: Rat WIF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for WIF1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G29-W379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WIF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat WIF1 amount of sample captured in plate.
Background: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Principle: Rat WIF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for WIF1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G29-W379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WIF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat WIF1 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,063 Da
NCBI Official Full Name
wnt inhibitory factor 1
NCBI Official Synonym Full Names
Wnt inhibitory factor 1
NCBI Official Symbol
Wif1
NCBI Protein Information
wnt inhibitory factor 1
UniProt Protein Name
Wnt inhibitory factor 1
Protein Family
UniProt Gene Name
Wif1
UniProt Synonym Gene Names
WIF-1
NCBI Description
human homolog has decreased mRNA expression in some prostate cancers and other cancers; may act as a secreted inhibitor of the Wnt signaling pathway [RGD, Feb 2006]
Uniprot Description
WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Protein type: Inhibitor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q22
Biological Process: positive regulation of fat cell differentiation
Research Articles on WIF1
Similar Products
Product Notes
The Rat WIF1 wif1 (Catalog #AAA178750) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178750 ELISA Kit recognizes Rat WIF1. It is sometimes possible for the material contained within the vial of "WIF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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