Mouse MMP-8 ELISA Kit | Mmp8 elisa kit

Mouse MMP-8 PicoKine ELISA Kit

Cat. Num. AAA178729

• Gene Names: Mmp8; BB138268
• Reactivity: Mouse
• Synonyms: MMP-8; Mouse MMP-8 PicoKine ELISA Kit; CLG1; Matrix metalloproteinase-8; MMP8; P22894; PMNL CL; PMNL collagenase; PMNLCL; PMNL-CLCL; Neutrophil collagenase; matrix metallopeptidase 8; Mmp8 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse MMP-8
• Sequence Length: 465

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin
• TMB incubation time: 15-20
• Immunogen: NSO, F21-S465
• Sample Data: Concentration(pg/ml): |0|156|312|625|1250|2500|5000|10000; O.D.: |0.009|0.055|0.126|0.246|0.499|0.907|1.680|2.425
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |199|1050|4121|254|926|3669; Standard Deviation: |8.56|54.45|173.08|14.73|70.37|190.79; CV(%): |4.3|4.9|4.2|5.8|7.6|5.2
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse MMP-8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin) to each empty well. It is recommended that each mouse MMP-8 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse MMP-8 PicoKine ELISA Kit standard curve )

Related Product Information for Mmp8 elisa kit

For quantitative detection of mouse MMP-8 in cell culture supernates, serum and plasma (heparin).
Background: Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.
Principle: Mouse MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for MMP-8 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: F21-S465) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MMP-8 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 160333381
• NCBI GeneID: 17394
• NCBI Accession #: NP_032637.3
• NCBI GenBank Nucleotide #: NM_008611.4
• UniProt Accession #: O70138; O88733; Q6GTR5
• Molecular Weight: 53,126 Da
• NCBI Official Full Name: neutrophil collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: Mmp8
• NCBI Official Synonym Symbols: BB138268
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: Mmp8
• UniProt Synonym Gene Names: MMP-8

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades types I, II and III collagens. Mice lacking the encoded protein exhibit abnormalities in the inflammatory responses to various agents. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.34; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9|9 A1

Cellular Component: extracellular space

Molecular Function: calcium ion binding; metallopeptidase activity; serine-type endopeptidase activity; zinc ion binding

Biological Process: proteolysis