Rat CD44 PicoKine ELISA Kit | Cd44 elisa kit

Rat CD44 PicoKine ELISA Kit

Cat. Num. AAA177555

• Gene Names: Cd44; CD44A; METAA; RHAMM
• Reactivity: Rat
• Synonyms: CD44 PicoKine; Rat CD44 PicoKine ELISA Kit; CD44 antigen; LHR; BA-1; CD 44; CD44; CD44 molecule(Indian blood group); CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen ; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; IN; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH1; PGP-1; PGP-I; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; Cd44 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CD44
• Sequence Length: 364

• Assay Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of rat CD44 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO,Q22-E271
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20,000pg/ml, 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml rat CD44 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat CD44 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|1.76|5.5|12.8|2.1|7.6|14; Standard deviation|0.09|0.35|0.73|0.16|0.52|0.88; CV(%)|5.1|6.4|5.7|7.8|6.9|6.3
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10,000|20,000; O.D.|0.005|0.118|0.224|0.378|0.702|1.346|1.694|2.178
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat CD44 PicoKine ELISA Kit standard curve )

Related Product Information for Cd44 elisa kit

Principle of the assay: Rat CD44 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD44 has been precoated onto 96-well plates. Standards(NSO, Q22 - E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD44 amount of sample captured in plate.

Background: CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 564342041
• NCBI GeneID: 25406
• NCBI Accession #: XP_006234693.1
• NCBI GenBank Nucleotide #: XM_006234631.2
• UniProt Accession #: P26051; Q99021
• Molecular Weight: 39,794 Da
• NCBI Official Full Name: CD44 antigen isoform X7
• NCBI Official Synonym Full Names: CD44 molecule (Indian blood group)
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: CD44A; METAA; RHAMM
• NCBI Protein Information: CD44 antigen
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: ECMR-III; PGP-1; PGP-I
• UniProt Entry Name: CD44_RAT

NCBI Description

adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD, Feb 2006]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Receptor, misc.

Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; cytoplasm; external side of plasma membrane; focal adhesion; Golgi apparatus; integral to membrane; nucleus; plasma membrane; protein complex

Molecular Function: cytokine binding; epidermal growth factor receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; hyaluronic acid binding; hyalurononglucosaminidase activity; phosphoprotein binding; protein binding; protein kinase binding; punt binding

Biological Process: blood vessel maturation; cartilage development; cell adhesion; cell migration; cytokine and chemokine mediated signaling pathway; healing during inflammatory response; hyaluronan catabolic process; inflammatory response; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of inflammatory response; negative regulation of mature B cell apoptosis; negative regulation of regulatory T cell differentiation; neurite development; positive regulation of heterotypic cell-cell adhesion; positive regulation of kinase activity; positive regulation of neutrophil apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell growth; response to organic cyclic substance; response to vitamin A; ureteric bud branching; Wnt receptor signaling pathway