Mouse ALCAM PicoKine ELISA Kit | Alcam elisa kit

Mouse ALCAM PicoKine ELISA Kit

Cat. Num. AAA177556

• Gene Names: Alcam; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• Reactivity: Mouse
• Synonyms: ALCAM PicoKine; Mouse ALCAM PicoKine ELISA Kit; CD166 antigen; Activated leukocyte cell adhesion molecule; ALCAM; ALCAM protein; CD 166; CD166; CD166_HUMAN; FLJ3851; FLJ38514; MEMD; MGC71733; activated leukocyte cell adhesion molecule; Alcam elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse ALCAM
• Sequence Length: 583

• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse ALCAM in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, W28-K527
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse ALCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ALCAM standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|0.57|2.33|4.25|0.68|2.74|4.61; Standard deviation|0.029|0.086|0.136|0.058|0.17|0.244; CV(%)|5.1|3.7|3.2|8.6|6.2|5.3
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.066|0.098|0.164|0.294|0.544|1.007|1.821|2.388
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse ALCAM PicoKine ELISA Kit standard curve )

Related Product Information for Alcam elisa kit

Principle of the assay: Mouse ALCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ALCAM has been precoated onto 96-well plates. Standards(NSO, W28-K527) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ALCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ALCAM amount of sample captured in plate.

Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

NCBI and Uniprot Product Information

• NCBI GI #: 31791059
• NCBI GeneID: 11658
• NCBI Accession #: NP_033785.1
• NCBI GenBank Nucleotide #: NM_009655.2
• UniProt Accession #: Q61490; O70136; Q8CDA5; Q8R2T0
• Molecular Weight: 65,092 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Official Synonym Symbols: BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam
• UniProt Entry Name: CD166_MOUSE

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: axon; cell projection; cell soma; external side of plasma membrane; focal adhesion; immunological synapse; integral to membrane; intrinsic to plasma membrane; membrane; plasma membrane; T cell receptor complex

Molecular Function: protein binding

Biological Process: adaptive immune response; axon guidance; cell adhesion; heterophilic cell adhesion; immune system process; motor axon guidance