Goat anti-Rat Oncomodulin Polyclonal Antibody | anti-Ocm2 antibody

Oncomodulin (OM, parvalbumin beta)

Cat. Num. AAA6012960

• Gene Names: Ocm2; OM; Ocm; oncmodulin
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Oncomodulin; Polyclonal Antibody; Oncomodulin (OM; parvalbumin beta); Anti -Oncomodulin (OM; anti-Ocm2 antibody

• Host: Goat
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Oncomodulin. Species sequence homology: Recombinant human Oncomodulin (40%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Applicable Applications for anti-Ocm2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa1-109 from rat Oncomodulin expressed in E. coli (P02631).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Rat Oncomodulin by Western Blot. Western blot shows lysates of rat placenta tissue. PVDF Membrane was probed with 1ug/ml of Rat Oncomodulin Antigen Affinity­purified Polyclonal Antibody followed by HRP­ conjugated Goat IgG Secondary Antibody. A specific band was detected for Oncomodulin at approximately 12kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)

Related Product Information for anti-Ocm2 antibody

Oncomodulin is a 12-14kD member of the parvalbumin family of Ca++binding proteins. It is expressed in early embryonic cells, placenta, and in tumors. OM was originally thought to have expression restricted to neoplastic tissues, early embryonic cells and certain tumor cell lines. Recent research shows that oncomodulin is also expressed and secreted by macrophages where, in the retina, it binds to retinal ganglion cells (RGCs) and functions to promote axon regenerationin early embryonic cells, placenta, and in tumors. OM is both cytoplasmic, and secreted. Rat OM is 109aa in length. It contains a vestigial Ca++binding site 733aa and two EF hand domains, the latter of which contains one highaffinity Ca++binding site 78108aa. Relative to parvalbumin alpha, OM has a lower pI (<4.8), a higher affinity for Ca++, and they share only 50aa identity. Full length rat OM shares 95-89aa identity with mouse and human OM, respectively.

Product Categories/Family for anti-Ocm2 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 39930606
• NCBI GeneID: 25503
• NCBI Accession #: NP_037127.1
• NCBI GenBank Nucleotide #: NM_012995.1
• UniProt Accession #: P02631
• Molecular Weight: 12,188 Da
• NCBI Official Full Name: oncomodulin
• NCBI Official Synonym Full Names: oncomodulin 2
• NCBI Official Symbol: Ocm2
• NCBI Official Synonym Symbols: OM; Ocm; oncmodulin
• NCBI Protein Information: oncomodulin; parvalbumin beta
• UniProt Protein Name: Oncomodulin
• Protein Family: Oncomodulin
• UniProt Gene Name: Ocm
• UniProt Synonym Gene Names: OM
• UniProt Entry Name: ONCO_RAT

NCBI Description

calcium-binding protein; expression activated in a variety of tumors [RGD, Feb 2006]

Uniprot Description

Function: Has some calmodulin-like activity with respect to enzyme activation and growth regulation. Binds two calcium ions.

Tissue specificity: Found in tumor tissues and not detected in normal tissues.

Sequence similarities: Belongs to the parvalbumin family.Contains 2 EF-hand domains.