Western Blot (WB)
(Detection of Human EGLN2/PHD1 by Western Blot. Western blot shows lysates of MDAMB231 human breast cancer cell line. PVDF Membrane was probed with 1ug/ml of Human EGLN2/PHD1 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody. A specific band was detected for EGLN2/PHD1 at approximately 48kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Sheep anti-Human PHD1 Polyclonal Antibody | anti-LOC100170700 antibody
PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine (C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HI
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PHD1; Polyclonal Antibody; PHD1 (Prolyl Hydroxylase Domain-containing Protein 1; PHD-1; DKFZp434E026; EGL Nine (C.elegans) Homolog 2; Egl Nine Homolog 2; EGLN2; Estrogen-induced Tag 6; EIT6; EIT-6; Hypoxia-inducible Factor Prolyl Hydroxylase 1; HIF Prolyl Hydroxylase 1; HIF-PH1; HI; Anti -PHD1 (Prolyl Hydroxylase Domain-containing Protein 1; anti-LOC100170700 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EGLN2/PHD1.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS with Trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-LOC100170700 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1ug/ml
Immunoprecipitation: 25ug/ml
Dilution: Western Blot: 1ug/ml
Immunoprecipitation: 25ug/ml
Immunogen
Recombinant corresponding to S. frugiperda insect ovarian cell line human EGLN2/PHD1 expressed in Sf 21 cells (Q96KS0).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human EGLN2/PHD1 by Western Blot. Western blot shows lysates of MDAMB231 human breast cancer cell line. PVDF Membrane was probed with 1ug/ml of Human EGLN2/PHD1 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody. A specific band was detected for EGLN2/PHD1 at approximately 48kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Western Blot (WB)
(Detection of Human EGLN2/PHD1 by Western Blot. Western blot shows lysates of MDAMB231 human breast cancer cell line. PVDF Membrane was probed with 1ug/ml of Human EGLN2/PHD1 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody. A specific band was detected for EGLN2/PHD1 at approximately 48kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Related Product Information for anti-LOC100170700 antibody
EGLN2 (EGL/EGg LayingNine #2 also known as PHD1 and HPH3) is a 43kD member of the EglN family of proteins. It is ubiquitously expressed and found principally in the nucleus. EGLN2 hydroxylates proline on HIF1 a. HIF1 is an a/b heterodimeric transcriptional activa that up
Product Categories/Family for anti-LOC100170700 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
PHD1
NCBI Official Symbol
LOC100170700
NCBI Protein Information
PHD1
Similar Products
Product Notes
The LOC100170700 (Catalog #AAA6009619) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine (C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HI reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1ug/ml Immunoprecipitation: 25ug/ml. Researchers should empirically determine the suitability of the LOC100170700 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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