Western Blot (WB)
(Detection of Human SMC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5ug/ml of human SMC1 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated AntiGoat IgG Secondary Antibody. A specific band was detected for SMC1 at approximately 145kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Goat SMC1 Polyclonal Antibody | anti-SMC1 antibody
SMC1 (Structural Maintenance Of ChromosomeS Protein 1A, SMC Protein 1A, SMC-1A, SMC-1-alpha, Sb1.8, SMC1A, DXS423E, KIAA0178, SB1.8, SMC1L1)
Gene Names
SMC1; CG6057; DmelCG6057; DmSMC1; dSMC1; smc1; Smc1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SMC1; Polyclonal Antibody; SMC1 (Structural Maintenance Of ChromosomeS Protein 1A; SMC Protein 1A; SMC-1A; SMC-1-alpha; Sb1.8; SMC1A; DXS423E; KIAA0178; SB1.8; SMC1L1); Anti -SMC1 (Structural Maintenance Of ChromosomeS Protein 1A; anti-SMC1 antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SMC1. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-SMC1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.5ug/ml
Dilution: Western Blot: 0.5ug/ml
Immunogen
E.coli-derived recombinant human SMC1, Met837-Gln1233 (Accession # Q14683.2).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human SMC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5ug/ml of human SMC1 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated AntiGoat IgG Secondary Antibody. A specific band was detected for SMC1 at approximately 145kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Human SMC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5ug/ml of human SMC1 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated AntiGoat IgG Secondary Antibody. A specific band was detected for SMC1 at approximately 145kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Related Product Information for anti-SMC1 antibody
Structural Maintenance of Chromosome 1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a Vshaped molecule with two long coiledcoil arms, each having an ATPbinding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966.
Product Categories/Family for anti-SMC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
SMC1
NCBI Official Symbol
SMC1
NCBI Official Synonym Symbols
CG6057; DmelCG6057; DmSMC1; dSMC1; smc1; Smc1
NCBI Protein Information
CG6057 gene product from transcript CG6057-RA; SMC1; CG6057-PA; SMC1-PA; Smc1 cohesin subunit; cohesin subunit SMC1; cohesin complex subunit SMC1
Research Articles on SMC1
Similar Products
Product Notes
The SMC1 (Catalog #AAA626509) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SMC1 (Structural Maintenance Of ChromosomeS Protein 1A, SMC Protein 1A, SMC-1A, SMC-1-alpha, Sb1.8, SMC1A, DXS423E, KIAA0178, SB1.8, SMC1L1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.5ug/ml. Researchers should empirically determine the suitability of the SMC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
