Western Blot (WB)
(Detection of Mouse Semaphorin 4G by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1ug/ml of Mouse Semaphorin 4G Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4G at approximately 110kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Sheep anti-Human, Mouse Semaphorin 4G Polyclonal Antibody | anti-SEMA4G antibody
Semaphorin 4G (SEMA4G, KIAA1619)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Western Blot (WB)
(Detection of Mouse Semaphorin 4G by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1ug/ml of Mouse Semaphorin 4G Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4G at approximately 110kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Mouse Semaphorin 4G by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1ug/ml of Mouse Semaphorin 4G Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4G at approximately 110kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
NCBI and Uniprot Product Information
NCBI Description
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
SEMA4G: a single-pass type I membrane protein that may play a role in axon guidance.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q24.31
Cellular Component: plasma membrane; integral to membrane
Molecular Function: receptor activity
Biological Process: nervous system development; cell differentiation
Research Articles on SEMA4G
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Product Notes
The SEMA4G sema4g (Catalog #AAA6006320) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Semaphorin 4G (SEMA4G, KIAA1619) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the SEMA4G sema4g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 4G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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