Typical Testing Data/Standard Curve (for reference only)
Sheep D4R/DRD4 ELISA Kit | D4R/DRD4 elisa kit
Sheep D4R/DRD4 (Dopamine Receptor D4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to D4R/DRD4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for D4R/DRD4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain D4R/DRD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of D4R/DRD4. You can calculate the concentration of D4R/DRD4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Cellular Component: integral to plasma membrane; dendrite; dendritic spine; integral to membrane; terminal button; cell cortex; vesicle membrane; cell soma; membrane; axon; cytoplasm; plasma membrane; nerve terminal
Molecular Function: dopamine D2 receptor-like receptor activity; G-protein coupled receptor activity; identical protein binding; signal transducer activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding
Biological Process: synaptic transmission, dopaminergic; fear response; behavioral fear response; activation of MAPK activity; short-term memory; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of kinase activity; rhythmic process; regulation of calcium-mediated signaling; signal transduction; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; associative learning; regulation of synaptic transmission; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of dopamine metabolic process; regulation of circadian rhythm; arachidonic acid secretion; G-protein coupled receptor protein signaling pathway; olfactory learning; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity
Research Articles on D4R/DRD4
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Product Notes
The Sheep D4R/DRD4 drd4 (Catalog #AAA2506942) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506942 ELISA Kit recognizes Sheep D4R/DRD4. It is sometimes possible for the material contained within the vial of "D4R/DRD4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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