Western Blot (WB)
(Sample Data: Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 kDa Parkin protein. The immunolabeling is absent in the Parkin S101 mutant.)
Rabbit Parkin Polyclonal Antibody
Anti-phospho-Parkin (Ser101)
Reactivity
Human, Bovine, non-Human Primate
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
Parkin; Polyclonal Antibody; Anti-phospho-Parkin (Ser101); anti-Parkin antibody
Host
Rabbit
Reactivity
Human, Bovine, non-Human Primate
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the Parkin protein phosphorylated at serine 101
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-Parkin antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser101 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 kDa Parkin protein. The immunolabeling is absent in the Parkin S101 mutant.)
Western Blot (WB)
(Sample Data: Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 kDa Parkin protein. The immunolabeling is absent in the Parkin S101 mutant.)
Related Product Information for anti-Parkin antibody
The Parkin protein is a component of the E3 ubiquitin ligase complex, which is responsible for targeting of proteins for degradation. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene. The disease is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (1). Recent studies indicate that phosphorylation of Parkin at Ser101 and Ser378 might be important for regulating E3 ubiquitin ligase activity (2).
Product Categories/Family for anti-Parkin antibody
References
1. Jenner P. et al: Oxidative stress as a cause of nigral cell death in Parkinson's disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7, 1992.
2. Yamamoto A. et al: Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol Chem. 280(5):3390-9, 2005.
2. Yamamoto A. et al: Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol Chem. 280(5):3390-9, 2005.
Similar Products
Product Notes
The Parkin (Catalog #AAA516133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-Parkin (Ser101) reacts with Human, Bovine, non-Human Primate and may cross-react with other species as described in the data sheet. AAA Biotech's Parkin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the Parkin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parkin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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