Western Blot (WB)
(Sample Data: Western blot of HeLa lysate showing specific immunolabeling of the ~90kDa RSK3 protein.)
Rabbit RSK3 Polyclonal Antibody | anti-RSK3 antibody
Anti-RSK3
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
RSK3; Polyclonal Antibody; Anti-RSK3; anti-RSK3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the RSK3 protein
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-RSK3 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of HeLa lysate showing specific immunolabeling of the ~90kDa RSK3 protein.)
Western Blot (WB)
(Sample Data: Western blot of HeLa lysate showing specific immunolabeling of the ~90kDa RSK3 protein.)
Related Product Information for anti-RSK3 antibody
The p90 ribosomal S6 kinases (RSK)1-4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. RSK3 encodes a 733-amino-acid protein with a unique N-terminal region, which contains a putative nuclear localization signal (1). RSK3 mRNA is widely expressed and is activated by growth factors, serum and phorbol esters. Upon stimulation, RSK3 translocates to the cell nucleus and phosphorylates nuclear proteins. RSK3 can bind to ERK1/2 and this association increases the duration of RSK3 activation (2).
Product Categories/Family for anti-RSK3 antibody
References
1. Zhao, Y. et al: RSK3 encodes a novel pp90rsk isoform with a unique N-terminal sequence: growth factor-stimulated kinase function and nuclear translocation. Mol Cell Biol. 1995 Aug;15(8):4353-63.
2. Roux, P. P. et al: Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol. 2003 Jul;23(14):4796-804.
2. Roux, P. P. et al: Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol. 2003 Jul;23(14):4796-804.
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Product Notes
The RSK3 (Catalog #AAA516187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RSK3 reacts with Human, Mouse, Rat, Bovine, Canine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's RSK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the RSK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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