Rabbit ELK1 Polyclonal Antibody | anti-phospho-ELK1 antibody
Anti-phospho-ELK1 (Ser383)
Reactivity
Human, Mouse, Rat, Canine, non-Human Primate, Zebrafish
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
ELK1; Polyclonal Antibody; Anti-phospho-ELK1 (Ser383); anti-phospho-ELK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Canine, non-Human Primate, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ELK1 protein phosphorylated at serine 383
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-ELK1 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of recombinant ELK1 showing specific immunolabeling of the ~46kDa ELK1 phosphorylated at Ser383. The immunolabeling is absent in dephospho- ELK1.)
Related Product Information for anti-phospho-ELK1 antibody
ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 interacts with the promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). MAP kinase phosphorylates ELK1 at Ser383 to control the transcriptional activity of ELK1 (2). Reduced phosphorylation of ELK1 is implicated in premature aging syndrome and insulin resistance (3).
Product Categories/Family for anti-phospho-ELK1 antibody
References
1. Chen, A G. et al: Overexpression of Ets-like protein 1 in human esophageal squamous cell carcinoma. World J Gastroenerol. 2006. 28;12(48):7859-63.
2. Li, Q J. et al: MAP kinase phosphorylation dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 2003 22:281-291.
3. Knebel, B B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.
2. Li, Q J. et al: MAP kinase phosphorylation dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 2003 22:281-291.
3. Knebel, B B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.
