Western Blot (WB)
(Sample Data: Western blot of recombinant ELK1 showing specific immunolabeling of the ~46kDa ELK1 phosphorylated at Ser383. The immunolabeling is absent in dephospho- ELK1.)
Rabbit ELK1 Polyclonal Antibody | anti-phospho-ELK1 antibody
Anti-phospho-ELK1 (Ser383)
Reactivity
Human, Mouse, Rat, Canine, non-Human Primate, Zebrafish
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
ELK1; Polyclonal Antibody; Anti-phospho-ELK1 (Ser383); anti-phospho-ELK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Canine, non-Human Primate, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ELK1 protein phosphorylated at serine 383
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-ELK1 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of recombinant ELK1 showing specific immunolabeling of the ~46kDa ELK1 phosphorylated at Ser383. The immunolabeling is absent in dephospho- ELK1.)
Western Blot (WB)
(Sample Data: Western blot of recombinant ELK1 showing specific immunolabeling of the ~46kDa ELK1 phosphorylated at Ser383. The immunolabeling is absent in dephospho- ELK1.)
Related Product Information for anti-phospho-ELK1 antibody
ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 interacts with the promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). MAP kinase phosphorylates ELK1 at Ser383 to control the transcriptional activity of ELK1 (2). Reduced phosphorylation of ELK1 is implicated in premature aging syndrome and insulin resistance (3).
Product Categories/Family for anti-phospho-ELK1 antibody
References
1. Chen, A G. et al: Overexpression of Ets-like protein 1 in human esophageal squamous cell carcinoma. World J Gastroenerol. 2006. 28;12(48):7859-63.
2. Li, Q J. et al: MAP kinase phosphorylation dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 2003 22:281-291.
3. Knebel, B B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.
2. Li, Q J. et al: MAP kinase phosphorylation dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 2003 22:281-291.
3. Knebel, B B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.
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Product Notes
The phospho-ELK1 (Catalog #AAA515956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-ELK1 (Ser383) reacts with Human, Mouse, Rat, Canine, non-Human Primate, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ELK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-ELK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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