Rabbit p38 alpha Polyclonal Antibody | anti-phospho-p38alpha antibody

Anti-phospho-p38 alpha (Thr180 Tyr182)

Cat. Num. AAA516100

• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Zebrafish
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Chromatography
• Synonyms: p38 alpha; Polyclonal Antibody; Anti-phospho-p38 alpha (Thr180 Tyr182); anti-phospho-p38alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Zebrafish
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the p38 alpha protein phosphorylated at threonine 180 and tyrosine 182
• Purity/Purification: Affinity Chromatography
• Form/Format: 100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.

• Applicable Applications for anti-phospho-p38alpha antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1,000; IHC:1:250
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39kDa p38 alpha protein phosphorylated at Thr180/Tyr182.)

Immunostaining

(Sample Data: Immunostaining of human breast cancer tissue showing p38 alpha protein when phosphorylated at Thr180/Tyr182 in red. Photo courtesy of Patsy Ruegg.)

Related Product Information for anti-phospho-p38alpha antibody

p38alpha (a.k.a. SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or by autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include the transcriptional regulators ATF2, MEF2C, MAX, the cell cycle regulator CDC25B, and the tumor suppressor p53. Its wide range of targets suggest that p38 has roles in stress related transcription, cell cycle regulation and genotoxic stress response. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (3).

Product Categories/Family for anti-phospho-p38alpha antibody

Phospho Specific Antibodies; Angiogenesis; Apoptosis/Autophagy; Cardiovascular Disease; Cellular Stress; Inflammation; Metabolic Disorder; Neurobiology; NfkB Pathway; p38 Pathway; Ser/Thr Kinases

References

1. Han, J. et al: A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 265: 808-811, 1994.
2. Ge, B. et al: MAPKK-independent activation of p38-alpha mediated by TAB1-dependent autophosphorylation of p38-alpha. Science 295: 1291-1294, 2002.
3. Lin A. et al: Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. Science 268:286-290, 1995.